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Unsuitability of the assay for cell-mediated lympholysis in inbred mice for H-Y antigen determination of human cells

Summary

This study examined the H-Y-specific in vitro restimulation of splenocytes from in vivo intraperitoneally (i.p.) primed C57B1/6 (B6) female mice. In vivo priming was carried out with human male or female fibroblasts or peripheral blood lymphocytes, respectively. It was attempted to measure the in vitro H-Y-specific activity by cell-mediated lympholysis and by cell proliferation. 3[H]Thymidine incorporation was determined in mixed lymphocyte cultures (MLCs) of xenogenetic primed female splenocytes (responder cells) and of syngeneic lethally irradiated male splenocytes (stimulator cells). The xenogenic H-Y presentation by in vivo sensitization did not induce in the in vitro restimulation system an H-Y-specific cell proliferation or in the effector phase the generation of H-Y-specific killer cells. The assay for celimediated lympholysis and lymphocyte proliferation after xenogeneic priming and syngeneic in vitro restimulation is, thus, not suitable for H-Y testing of human cells.

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References

  1. Biasi G, Dazzi F, Loveland B, Rivarrollo R, Asherson G (1985) The failure of female cells to present in vitro the male H-Y antigen for secondary cytotoxic T-cell response. Immunogenetics 22:177–181

  2. Billingham RE, Silvers WK (1960) Studies on tolerance of the Y-chromosome antigen in mice. J Immunol 85:14–26

  3. Böhmer H von, Turton K, Haas W (1979) The role of the left end of the H-2b haplotype in the male-specific cytotoxic T cell response. Eur J Immunol 9:913–915

  4. Eichwald EJ, Silmser CR (1955) Untitled communication. Transplant Bull 2:148–149

  5. Fierz W, Brenan A, Müllbacher A, Simpson E (1982a) Non-H-2 and H-2-linked immune response genes control the cytotoxic T-cell response to H-Y. Immunogenetics 15:261–270

  6. Fierz W, Farmer GA, Sheena JH, Simpson E (1982b) Genetic analysis of the non-H-2-linked Ir genes controlling the cytotoxic T-cell response to H-Y in H-2d mice. Immunogenetics 16:593–601

  7. Gasser DL, Silver WK (1972) Genetics and immunology of sex-linked antigens. Adv Immunol 15:215–247

  8. Goldberg EH, Shen FW, Tokuda S (1973) Detection of H-Y (male) antigen on mouse lymph node cells by cell to cell cytotoxicity test. Transplantation 15:334–336

  9. Gordon RD, Simpson E, Samelson LE (1975) In vitro cell-mediated immune responses to the male specific (H-Y) antigen in mice. J Exp Med 142:1108–1120

  10. Gordon RD, Mathieson BJ, Samelson LE, Boyse EA, Simpson E (1976) The effect of allogeneic presentation on H-Y graft survival and in vitro cell-mediated responses to H-Y antigen. J Exp Med 144:810–820

  11. Goulmy E, Blokland E, van Rood J, Charmot D, Malissen B, Mawas C (1980) Production, expansion, and clonal analysis of T cells with specific HLA-restricted male lysis. J Exp Med 152:182–190

  12. Goulmy E, van Leeuween A, Blokland E, Sachs ES, Geraedts JPM (1983) The recognition of abnormal sex chromosome constitution by HLA restricted anti-H-Y cytotoxic T cells and antibody. Immunogenetics 17:523–531

  13. Hurme M, Hetherington CM, Chandler PR, Simpson E (1978a) Cytotoxic T-cell responses to H-Y: mapping of the Ir genes. J Exp Med 147:758–767

  14. Hurme M, Chandler PR, Hetherington CM, Simpson E (1978b) Cytotoxic T-cell responses to H-Y: correlation with the rejection of syngeneic male skin grafts. J Exp Med 147:768–775

  15. Juretié A, Malenica B, Juretié E, Klein J, Nagy ZA (1985) Helper effects required during in vivo priming for a cytologic response to the H-Y antigen in nonresponder mice. J Immunol 134:1408–1414

  16. Klein J (1986) How many class II immune response genes? A commentary on a reappraisal. Immunogenetics 23:309–310

  17. Liew FY, Simpson E (1980) Delayed-type hypersensitivity responses to H-Y: characterisation and mapping of the Ir genes. Immunogenetics 11:255–266

  18. Matsunaga T, Simpson E (1978) H-2 complementation in anti-H-Y cytotoxic T-cell responses can occur in chimeric mice. Proc Natl Acad Sci 75:6207–6210

  19. Müllbacher A, Brenan M (1980) Cytotoxic T-cell response to H-Y in “non-responder” CBA mice. Nature 285:34–36

  20. Pfeffer PF, Sodal G, Thorsby E (1981) HLA-restricted cell-mediated cytotoxicity directed against male specific (H-Y) antigen after acute rejection of an HLA-identical sibling kidney. Scand J Urol Nephrol [Suppl] 64:72–78

  21. Silvers WK, Yang SL (1973) Male specific antigen: homology in mice and rats. Science 181:570–572

  22. Simpson E, Gordon RD (1977) Responsiveness to H-Y antigen: Ir gene complementation and target cell specificity. Immunol Rev 35:59–75

  23. Simpson E, Liebermann R, Andó I, Sachs DH, Paul WE, Berzofsky JA (1986a) How many class II immune response genes? A reappraisal of the evidence. Immunogenetics 23:302–308

  24. Simpson E, Liebermann R, Andó I, Sachs DH, Paul WE, Berzofsky JA (1986b) A comment on the commentary. Immunogenetics 23: 311

  25. Waal LP de, Hoop de J, Stukart MJ, Gleichmann H, Melvold RW, Melief CJ (1983a) Nonresponsiveness to the male antigen H-Y in H-2 I-A-mutant B6.C-H-2bm 12 is not caused by defective antigen presentation. J Immunol 130:655–660

  26. Waal LP de, Melvold RW, Melief CJ (1983b) Cytotoxic T lymphocyte nonresponsiveness to the male antigen H-Y in the H-2Db mutants bm13 and bm14. Complementation of the response in F1 crosses with the I-Ab mutant bm12 nonresponder and failure of B6 or Db mutant mice tolerant of each other to respond to allogeneic male cells. J Exp Med 158:1537–1546

  27. Wiberg UH (1985) H-Y transplantation antigen in human XO females. Hum Genet 69:15–18

  28. Wiberg UH, Fredga K (1985) The H-Y transplantation antigen is present in XO and X*X female wood lemmings (Myopus schisticolor). Immunogenetics 22:495–501

  29. Wiberg UH, Günther E (1985) Female wood lemmings with the mutant X*-chromosome carry the H-Y transplantation antigen. Immunogenetics 21:91–96

  30. Worley R, Jorgensen C, Eichwald EJ (1985) Limitations of a histologic assay for syn-, allo-, and xenogeneic H-Y antigen. J Reprod Immunol 8:121–128

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Correspondence to Andreas Braun.

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Braun, A., Cleve, H. Unsuitability of the assay for cell-mediated lympholysis in inbred mice for H-Y antigen determination of human cells. Hum Genet 76, 369–374 (1987). https://doi.org/10.1007/BF00272447

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Keywords

  • Cell Proliferation
  • Internal Medicine
  • Metabolic Disease
  • Human Cell
  • Thymidine