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Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures


Stable cell suspension cultures were established from two types of calli (one compact, nodular and embryogenic, the other friable and embryogenic) derived from cultured immature embryos of wheat (cv FLA302). Only aged calli, which had been subcultured for at least 5–8 months, formed suspensions comprised mainly of groups of small, round, densely cytoplasmic, starch-containing cells. Only the embryogenic suspension derived from the aged, compact and nodular callus formed distinct somatic embryos when plated on regeneration media containing IAA and zeatin. Upon subsequent transfer to fresh regeneration medium more than 200 green rooted plants were obtained.

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casein hydrolysate


2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid


indole-3-acetic acid


indole-3-butyric acid


Murashige and Skoog (1962) basal medium


naphthaleneacetic acid


packed cell volume


  1. Ahuja PS, Pental D, Cocking EC (1982) Z Pflanzenzuchtg 89:139–144

  2. Chen THU, Kartha KK, Gusta LV (1985) Pl Cell Tiss Organ Cult 4:101–109

  3. Chu CC, Wang CC, Sun CS (1975) Sci Sinica 18:659–668

  4. Dudits D, Hadlacky G, Levi E, Fejer O, Haydu Z, Lazar G (1977) Theoret Appl Genet 51:127–132

  5. Harris R, Wright M, Varnum J, Brightwell B, Schubert K (1988) Pl Cell Rep 7:337–340

  6. Karlsson SB, Vasil IK (1986) J Pl Physiol 123:211–227

  7. Klein TM, Roth BA, Fromm MR (1989) In: Setlow JK (ed) Genetic Engineering — Principles and Methods. Plenum, New York, pp 11:13–31

  8. Kyozuka J, Kaneda T, Shimamoto K (1989) Bio/Technology 7:1171–1174

  9. Maddock SE (1987) Pl Cell Rep 7:337–340

  10. Muller AJ, Grafe R (1978) Molec Gen Genet 161:67–76

  11. Murashige T, Skoog F (1962) Physiol Pl 15:473–497

  12. Potrykus I (1989) TIBTECH 7:269–273

  13. Redway, FA, Vasil V, Lu D, Vasil IK (1990) Theoret Appl Genet (In Press)

  14. Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ (1988) Science 240:204–207

  15. Vasil IK (1988) Bio/Technology 6:397–402

  16. Vasil V, Redway, F, Vasil IK (1990) Bio/Technology (In Press)

  17. Wang WC, Beyl C, Sharma GC (1983) Pl Sci Lett 31:147–156

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Author information

Correspondence to Indra K. Vasil.

Additional information

Communicated by E. D. Earle

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Redway, F.A., Vasil, V. & Vasil, I.K. Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures. Plant Cell Reports 8, 714–717 (1990).

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  • Cell Suspension
  • Somatic Embryo
  • Suspension Culture
  • Rooted Plant
  • Triticum Aestivum