Stable cell suspension cultures were established from two types of calli (one compact, nodular and embryogenic, the other friable and embryogenic) derived from cultured immature embryos of wheat (cv FLA302). Only aged calli, which had been subcultured for at least 5–8 months, formed suspensions comprised mainly of groups of small, round, densely cytoplasmic, starch-containing cells. Only the embryogenic suspension derived from the aged, compact and nodular callus formed distinct somatic embryos when plated on regeneration media containing IAA and zeatin. Upon subsequent transfer to fresh regeneration medium more than 200 green rooted plants were obtained.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
- GA3 :
Murashige and Skoog (1962) basal medium
packed cell volume
Ahuja PS, Pental D, Cocking EC (1982) Z Pflanzenzuchtg 89:139–144
Chen THU, Kartha KK, Gusta LV (1985) Pl Cell Tiss Organ Cult 4:101–109
Chu CC, Wang CC, Sun CS (1975) Sci Sinica 18:659–668
Dudits D, Hadlacky G, Levi E, Fejer O, Haydu Z, Lazar G (1977) Theoret Appl Genet 51:127–132
Harris R, Wright M, Varnum J, Brightwell B, Schubert K (1988) Pl Cell Rep 7:337–340
Karlsson SB, Vasil IK (1986) J Pl Physiol 123:211–227
Klein TM, Roth BA, Fromm MR (1989) In: Setlow JK (ed) Genetic Engineering — Principles and Methods. Plenum, New York, pp 11:13–31
Kyozuka J, Kaneda T, Shimamoto K (1989) Bio/Technology 7:1171–1174
Maddock SE (1987) Pl Cell Rep 7:337–340
Muller AJ, Grafe R (1978) Molec Gen Genet 161:67–76
Murashige T, Skoog F (1962) Physiol Pl 15:473–497
Potrykus I (1989) TIBTECH 7:269–273
Redway, FA, Vasil V, Lu D, Vasil IK (1990) Theoret Appl Genet (In Press)
Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ (1988) Science 240:204–207
Vasil IK (1988) Bio/Technology 6:397–402
Vasil V, Redway, F, Vasil IK (1990) Bio/Technology (In Press)
Wang WC, Beyl C, Sharma GC (1983) Pl Sci Lett 31:147–156
Communicated by E. D. Earle
About this article
Cite this article
Redway, F.A., Vasil, V. & Vasil, I.K. Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures. Plant Cell Reports 8, 714–717 (1990). https://doi.org/10.1007/BF00272101
- Cell Suspension
- Somatic Embryo
- Suspension Culture
- Rooted Plant
- Triticum Aestivum