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Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro

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Abstract

To develop an adventitious regeneration system for pear cultivars, several experiments were conducted with 2 cultivars of Pyrus communis L. (‘Seckel’ and ‘Louise Bonne’) and one cultivar of P. bretschneideri Rehd. (‘Crystal Pear’). Half-leaves, taken from shoots proliferating on Lepoivre medium, were plated in petri-dishes on medium supplemented with various combinations of cytokinins and auxins. Cultures of the above cultivars had been established from mature trees. Among the growth regulators tested, thidiazuron (TDZ), combined with naphthalene-acetic acid (NAA), was the most efficient for stimulation of adventitious shoots. The optimum level of TDZ was about 3 uM; shoot regeneration was observed over a wide range of TDZ and NAA concentrations (0.5 to 5 uM and 2.5 to 13 um, respectively). Among different macronutrient compositions, 1/2 and 1/4 Murashige and Skoog were the most effective. Sucrose concentrations (10 to 50 g L-1) had a linear significant effect on shoot regeneration of ‘Crystal Pear’.

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Abbreviations

BAP:

6-benzylaminopurine

IBA:

indole-3-butyric acid

NAA:

a-naphthaleneacetic acid

NoA:

2-naphthoxyacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

TDZ:

thidiazuron (N-phenyl-N'-1,2,3-thidiazol-5-ylurea)

MS:

Murashige and Skoog (1962) macroelements

L:

Lepoivre (Quoirin and Lepoivre, 1977) macroelements

References

  1. Druart P (1980) Sci Hort 12:339–342

  2. Evaldsson I (1985) Swedish J Agric Res 15:119–122

  3. Hutchinson JF, Zimmerman RH (1987) Hort Review 273–349

  4. James DJ, Mackenzie KAD, Mahlotra SB (1987) Theor Appl Genet 73:589–594

  5. James DJ, Passey AJ, Malhotra SB (1984) Plant Cell Tissue Organ Culture 3(4):333–341

  6. Jones OP, Gayner JA, Watkins R (1984) J Hort Sci 59(4):463–467

  7. Kerns HR, Meyer MM (1986) HortSci 21(5):1209–1210

  8. Mehra PN, Jaidka K (1979) Phytomorphology 29:286–298

  9. Mehra PN, Jaidka K (1985) Phytomorphology 35:1–9

  10. Murashige T, Skoog F (1962) Physiologia Pl 15:473–497

  11. Ochatt SJ, Caso OH (1986) J Plant Physiol 122:243–249

  12. Quoirin M, Lepoivre P (1977) Acta Hortic 78:437

  13. Revilla MA, Ochatt SJ, Doughty S, Power JB (1987) Plant Science, 50:133–137.

  14. Singha S (1986) Pear (Pyrus communis). In YPS Bajaj (ed.) Biotechnology in Agriculture and Forestry Vol. 1:Trees, Springer-Verlag Berlin, pp 198–206

  15. Steel RGD, Torrie J (1980) Principles and procedures of statistics. McGraw-Hill Book Company, pp 177–181

  16. van Nieuwkerk JP, Zimmerman RH, Fordham I (1986) HortScience 21(3):516–518

  17. Wanas WH, Callow JA, Withers (1986) In Withers LA and Alderson PG (eds.), Plant tissue culture and its agricultural applications, Butter-worths, London, pp 285–290

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Author information

Correspondence to R. M. Skirvin.

Additional information

Communicated by J.M. Widholm

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Chevreau, E., Skirvin, R.M., Abu-Qaoud, H.A. et al. Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro. Plant Cell Reports 7, 688–691 (1989). https://doi.org/10.1007/BF00272062

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Keywords

  • Sucrose
  • Growth Regulator
  • Leaf Tissue
  • Regeneration System
  • Optimum Level