Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Potential of Datura innoxia cell suspension cultures for glucosylating hydroquinone

  • 40 Accesses

  • 27 Citations


The efficiency of glucosylation of hydroquinone by Datura innoxia cell suspension cultures was investigated. The yield of arbutin was 2.4 g/l medium when 10 mM of hydroquinone was added to a suspension culture that was then incubated for 24 h, but the yield decreased at a higher concentration. This decrease, which could not be overcome by changing the growth phase or increasing the cell density used, could be avoided by the repeated addition of a low concentration of hydroquinone over 3 days. This increased the yield of arbutin to 4.2 g/l at the usual cell density and to 7.1 g/l at a high density. The kinetics of this reaction were explained by the Michaelis-Menten formula. The theoretical maximum velocity of the arbutin-forming reaction was estimated as 0.77 mg/h/g. The velocity increased linearly up to a cell density of 300 g/l under standard aeration, the theoretical maximum yield of arbutin being calculated to be 5.5 g/l/day.

This is a preview of subscription content, log in to check access.


  1. Döller PC, Alfermann AW, Reinhard E (1977) Planta Medica 31: 1–6

  2. Furuya T (1978) Biotransformation by plant cell cultures. In: Thorpe TA (ed) Frontiers of Plant Tissue Culture, University of Calgary Press, Calgary, pp 191–200

  3. Reinhard E, Alfermann AW (1980) Biotransformation by plant tissue cultures. In: Fiechter A (ed) Advances in Biochemical Engineering, Springer Verlag, Berlin, pp 49–83

  4. Tabata M, Ikeda F, Hiraoka N, Konoshima M (1976) Phytochemistry 15: 1225–1229

  5. Tabata M, Umetani Y, Shima K, Tanaka S (1984) Plant Cell Tissue Organ Culture 3: 3–9

Download references

Author information

Correspondence to Toshiyuki Suzuki.

Additional information

Communicated by F. Constabel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Suzuki, T., Yoshioka, T., Tabata, M. et al. Potential of Datura innoxia cell suspension cultures for glucosylating hydroquinone. Plant Cell Reports 6, 275–278 (1987). https://doi.org/10.1007/BF00271997

Download citation


  • Cell Suspension
  • Cell Density
  • Growth Phase
  • Suspension Culture
  • Maximum Yield