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Physical mapping of the restriction fragments obtained from bacteriophage T4 dC-DNA with the restriction endonucleases SmaI, KpnI and BglII


The cytosine-containing DNA of a mutant of bacteriophage T4 was digested with restriction endonucleases SmaI, KpnI and BglII producing 5, 7 and 13 fragments respectively. Complete physical maps of the T4 genome were constructed with the enzymes SmaI and KpnI and an almost complete map with the enzyme BglII.

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  1. Allet, B., Bukhari, A.I.: Analysis of bacteriophage Mu and λ-Mu hybrid DNAs by specific endonucleases. J. Mol. Biol. 92, 529–540 (1975)

  2. Allet, B., Jepessen, P.G.N., Katagiri, K.J., Delius, H.: Mapping the DNA fragments produced by cleavage of λ DNA with endonuclease RI. Nature 241, 120–123 (1973)

  3. Bautz, F.A., Bautz, E.K.F.: Mapping of deletions in a non-essential region of the phage T4 genome. J. Mol. Biol. 28, 345–355 (1967)

  4. Benzer, S.: On the topography of the genetic fine structure. Proc. Natl. Acad. Sci. U.S.A. 47, 403–426 (1961)

  5. Blin, N., Gabain, A. v., Bujard, H.: Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes. FEBS Lett. 53, 84–86 (1975)

  6. Champe, S.P.: Linkage map of bacteriophage T4. In: CRC Handbook of microbiology (Laskin, A.J. and Lechevalier, H.A., eds.), Vol. 4, pp. 605–608, Cleveland, Ohio: CRC Press 1974

  7. Endow, S.A., Roberts, R.J.: Two restriction-like enzymes from Xanthomonas malvacearum. J. Mol. Biol. 112, 521–529 (1977)

  8. Gabain, A. v., Hayward, G.S., Bujard, H.: Physical mapping of the HindIII, Eco RI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA. Mol. Gen. Genet. 143, 279–290 (1976)

  9. Greene, P.J., Betlach, M.C., Goodman, H.M., Boyer, H.W.: The Eco RI restriction endonuclease. In: DNA replication and biosynthesis, methods in molecular biology (Wickner, R.B., ed.), Vol. 7, pp. 87–111. New York: Marcel Decker 1974

  10. Lang, D.: Molecular weights of coliphages and coliphate DNA. J. Mol. Biol. 54, 557–565 (1970)

  11. Lehman, I.R., Pratt, E.A.: On the structure of the glucosylated hydroxymethylcytosine nucleotides of coliphages T2, T4, and T6. J. Biol. Chem. 235, 3254–3255 (1960)

  12. MacHattie, L.A., Ritchie, D.A., Thomas, C.A., Jr., Richardson, C.C.: Terminal repetition in permuted T2 bacteriophage molecules. J. Mol. Biol. 23, 355–363 (1967)

  13. Mattson, T., Houwe, G. Van, Bolle, A., Selzer, G., Epstein, R.: Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes. Mol. Gen. Genet. 154, 319–326 (1977)

  14. Rüger, W.: Transcription of bacteriophage T4 DNA in vitro: selective initiation with dinucleotides. Eur. J. Biochem. 88, 109–117 (1978)

  15. Selzer, G., Bolle, A., Krisch, H. Epstein, R.: Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4. Mol. Gen. Genet. 159, 301–309 (1978)

  16. Shaltiel, S., Er-El, Z.: Hydrophobic chromatography: use for purification of glycogen synthetase. Proc. Natl. Acad. Sci. U.S.A. 70, 778–781 (1973)

  17. Smith, H.O., Wilcox, K.W.: A restriction enzyme from Haemophilus influenzae. I. Purification and general properties. J. Mol. Biol. 51, 379–391 (1970)

  18. Snyder, L., Gold, L., Kutter, E.: A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA. Proc. Natl. Acad. Sci. U.S.A. 73, 3098–3102 (1976)

  19. Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)

  20. Streisinger, G., Edgar, R.S., Denhardt, G.H.: Chromosome structure in phage T4, I. Circularity of the linkage map. Proc. Natl. Acad. Sci. U.S.A. 51, 775–779 (1964)

  21. Tanaka, T., Weisblum, B.: Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of DNA. J. Bacteriol. 121, 354–362 (1975)

  22. Thomas, C.A., Jr.: The arrangement of information in DNA molecules. J. Gen. Physiol. 49/6, 2, 143–169 (1966)

  23. Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with Eco RI restriction endonuclease. J. Mol. Biol. 91, 315–328 (1975)

  24. Wyatt, G.R., Cohen, S.S.: A new pyrimidine base from bacteriophage nucleic acids. Nature 170, 1072–1073 (1952)

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Correspondence to Wolfgang Rüger.

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Communicated by H.G. Wittmann

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Kiko, H., Niggemann, E. & Rüger, W. Physical mapping of the restriction fragments obtained from bacteriophage T4 dC-DNA with the restriction endonucleases SmaI, KpnI and BglII. Molec. Gen. Genet. 172, 303–312 (1979). https://doi.org/10.1007/BF00271730

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  • Enzyme
  • Restriction Endonuclease
  • Restriction Fragment
  • Restriction Endonuclease SmaI
  • Enzyme BglII