Phage λimm 21 c spi − infecting recA − cells gives a burst of 6 progeny phages compared to 120 in rec + cells. Parental λspi − DNA is not degraded in recA − cells. The synthesis of early replication products is enhanced by a factor of 2 yielding 30 closed circular progeny DNA molecules per cell compared to 15 in the control. These DNA supercoils include 9% of dimer molecules under red − recA − and red − rec + conditions. On the other hand, the formation of linear phage DNA molecules in recA − cells is reduced by a factor of 5 to 6, if compared to λspi − DNA in rec + and λspi + DNA in recA − cells, respectively. The specific biological activity of these linear molecules in the helper phage assay system is unimpaired. Intermediates of late λspi − replication under recA − conditions are supposed to be the unprotected targets of the action of the recB + recC + nuclease.
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Bode, V.C., Kaiser, A.D.: Changes in the structure and activity of λDNA in a superinfected immune bacterium. J. molec. Biol. 14, 399–417 (1965).
Cohen, S.N., Chang, A.C.Y.: Genetic expression in bacteriophage λ, IV. Effects of P2-prophage on inhibition of host synthesis and λ gene expression. Virology 46, 397–406 (1971).
Hobom, G., Hogness, D.S.: The role of recombination in the formation of circular oligomers of the λdv plasmid. J. molec. Biol., in press (1972).
Ihler, G., Kawai, Y.: Alternate fate of the complementary strands of λDNA after infection of E. coli. J. molec. Biol. 61, 311–328 (1971).
Kaiser, A.D., Inman, R.B.: Cohesion and the biological activity of bacteriophage λDNA. J. molec. Biol. 13, 78–91 (1965).
Kiger, J.A., Sinsheimer, R.L.: DNA of vegetative Bacteriophage λ, VI. Electron microscopic studies of replicating λDNA. Proc. nat. Acad. Sci. (Wash.) 68, 112–115 (1971).
Lindahl, G., Sironi, G., Bialy, H., Calendar, R.: Bacteriophage λ; Abortive infection of bacteria lysogenic for phage P2. Proc. nat. Acad. Sci. (Wash.) 66, 587–594 (1970).
Lindqvist, B.H., Sinsheimer, R.L.: The process of infection with bacteriophage ϕ X 174, XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants J. molec. Biol. 30, 69–80 (1967).
Matsubara, K., Kaiser, A.D.: λdv: an autonomously replicating DNA fragment. Cold Spr. Harb. Symp. quant. Biol. 33, 768–775 (1968).
Nijkamp, H.J.J., Bøvre, K., Szybalski, W.: Controls of rightward transcription in coliphage λ. J. molec. Biol. 54, 599–604 (1970).
Nobrega, F.G., Rola, F.H., Pasetto-Nobrega, M., Oishi, M.: Adenosine triphosphatase associated with adenosine triphosphate dependant deoxyribonuclease. Proc. nat. Acad. Sci. (Wash.) 69, 15–19 (1972).
Sironi, G., Bialy, H., Lozeron, H.A., Calendar, R.: Bacteriophage P2: Interaction with phage λ and with recombination-deficient bacteria. Virology 46, 387–396 (1971).
Young II, E.T., Sinsheimer, R.L.: VegetativeλDNA, II. Physical characterization and replication. J. molec. Biol. 30, 165–200 (1967).
Young II, E.T., Sinsheimer, R.L.: Vegetative λDNA. III. Pulse-labeled components. J. molec. Biol. 33, 49–59 (1968).
Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage λ. Inhibition of growth by prophage P2. In: The bacteriophage λ (A.D. Hershey, ed) p. 469–475. Cold Spring Harbor, (N.Y.): Cold Spring Harbor Laboratories 1971a.
Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage λ. The γ gene. In: The bacteriophage λ (A.D. Hershey, ed.), p. 455–468. Cold Spring Harbor (N.Y.): Cold Spring Harbor Laboratories 1971b.
Communicated by P. Starlinger
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Hobom, B., Hobom, G. DNA-replication of spi − mutants of bacteriophage λ in recombination-deficient bacteria. Molec. Gen. Genet. 117, 229–238 (1972). https://doi.org/10.1007/BF00271650
- Biological Activity
- Assay System
- Linear Molecule
- Dime Molecule
- Early Replication