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DNA-replication of spi mutants of bacteriophage λ in recombination-deficient bacteria

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Summary

Phage λimm 21 c spi infecting recA cells gives a burst of 6 progeny phages compared to 120 in rec + cells. Parental λspi DNA is not degraded in recA cells. The synthesis of early replication products is enhanced by a factor of 2 yielding 30 closed circular progeny DNA molecules per cell compared to 15 in the control. These DNA supercoils include 9% of dimer molecules under red recA and red rec + conditions. On the other hand, the formation of linear phage DNA molecules in recA cells is reduced by a factor of 5 to 6, if compared to λspi DNA in rec + and λspi + DNA in recA cells, respectively. The specific biological activity of these linear molecules in the helper phage assay system is unimpaired. Intermediates of late λspi replication under recA conditions are supposed to be the unprotected targets of the action of the recB + recC + nuclease.

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References

  1. Bode, V.C., Kaiser, A.D.: Changes in the structure and activity of λDNA in a superinfected immune bacterium. J. molec. Biol. 14, 399–417 (1965).

  2. Cohen, S.N., Chang, A.C.Y.: Genetic expression in bacteriophage λ, IV. Effects of P2-prophage on inhibition of host synthesis and λ gene expression. Virology 46, 397–406 (1971).

  3. Hobom, G., Hogness, D.S.: The role of recombination in the formation of circular oligomers of the λdv plasmid. J. molec. Biol., in press (1972).

  4. Ihler, G., Kawai, Y.: Alternate fate of the complementary strands of λDNA after infection of E. coli. J. molec. Biol. 61, 311–328 (1971).

  5. Kaiser, A.D., Inman, R.B.: Cohesion and the biological activity of bacteriophage λDNA. J. molec. Biol. 13, 78–91 (1965).

  6. Kiger, J.A., Sinsheimer, R.L.: DNA of vegetative Bacteriophage λ, VI. Electron microscopic studies of replicating λDNA. Proc. nat. Acad. Sci. (Wash.) 68, 112–115 (1971).

  7. Lindahl, G., Sironi, G., Bialy, H., Calendar, R.: Bacteriophage λ; Abortive infection of bacteria lysogenic for phage P2. Proc. nat. Acad. Sci. (Wash.) 66, 587–594 (1970).

  8. Lindqvist, B.H., Sinsheimer, R.L.: The process of infection with bacteriophage ϕ X 174, XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants J. molec. Biol. 30, 69–80 (1967).

  9. Matsubara, K., Kaiser, A.D.: λdv: an autonomously replicating DNA fragment. Cold Spr. Harb. Symp. quant. Biol. 33, 768–775 (1968).

  10. Nijkamp, H.J.J., Bøvre, K., Szybalski, W.: Controls of rightward transcription in coliphage λ. J. molec. Biol. 54, 599–604 (1970).

  11. Nobrega, F.G., Rola, F.H., Pasetto-Nobrega, M., Oishi, M.: Adenosine triphosphatase associated with adenosine triphosphate dependant deoxyribonuclease. Proc. nat. Acad. Sci. (Wash.) 69, 15–19 (1972).

  12. Sironi, G., Bialy, H., Lozeron, H.A., Calendar, R.: Bacteriophage P2: Interaction with phage λ and with recombination-deficient bacteria. Virology 46, 387–396 (1971).

  13. Young II, E.T., Sinsheimer, R.L.: VegetativeλDNA, II. Physical characterization and replication. J. molec. Biol. 30, 165–200 (1967).

  14. Young II, E.T., Sinsheimer, R.L.: Vegetative λDNA. III. Pulse-labeled components. J. molec. Biol. 33, 49–59 (1968).

  15. Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage λ. Inhibition of growth by prophage P2. In: The bacteriophage λ (A.D. Hershey, ed) p. 469–475. Cold Spring Harbor, (N.Y.): Cold Spring Harbor Laboratories 1971a.

  16. Zissler, J., Signer, E., Schaefer, F.: The role of recombination in growth of bacteriophage λ. The γ gene. In: The bacteriophage λ (A.D. Hershey, ed.), p. 455–468. Cold Spring Harbor (N.Y.): Cold Spring Harbor Laboratories 1971b.

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Communicated by P. Starlinger

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Hobom, B., Hobom, G. DNA-replication of spi mutants of bacteriophage λ in recombination-deficient bacteria. Molec. Gen. Genet. 117, 229–238 (1972). https://doi.org/10.1007/BF00271650

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Keywords

  • Biological Activity
  • Assay System
  • Linear Molecule
  • Dime Molecule
  • Early Replication