Enhancing Factor (EF) is a 14 kDa protein isolated from mouse small intestines, which enhances the binding of 125I-EGF to A431 cells. This observation as well as our earlier in vitro studies have indicated that EF is a modulator of EGF. In adult mice, localization of EF by immunohistochemistry shows it is present pre-dominantly in the Paneth cells of the small intestines and to a lesser extent in the stomach and colon. This study of the ontogeny of EF shows that the appearance of the protein coincides with the appearance of mature Paneth cells. In new born mouse skin EF is localized in the hair follicles in the first hair cycle from day 2 to day 8. It is however absent in the adult skin. Thus EF is associated with tissues which have a high growth rate.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Ahonen A (1973) Histochemical and electron microscopic observations on the development, neural control and function of the Paneth cells of the mouse. Acta Physiologica Scand 398 [Suppl]
Cotsarelis G, Sun T-T, Lavker RM (1990) Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle and skin carcinogenesis. Cell 61:1329–1337
Creamer B (1967) Paneth cell function. Lancet I:314–316
Deo MG, Mulherkar R, Mane SM (1983) Isolation of a polypeptide that enhances cellular binding of epidermal growth factor. Ind J Biochem Biophys 20:228–231
Mulherkar R, Deo MG (1986a) Further studies on the enhancing factor and its possible mechanism of action. J Cell Physiol 127:183–188
Mulherkar R, Saraf A, Wagle A, Deo MG (1986b) Purification of the enhancing factor from mouse intestines. FEBS Lett 207:142–144
Mulherkar R, Desai SJ, Rao RS, Wagle AS, Deo MG (1991) Expression of Enhancing Factor gene and its localization in mouse tissues. Histochemistry 96:367–370
Pinkus H (1958) Embryology of hair. In: Montagana W, Ellis RA (eds) The biology of hair growth. Academic Press, New York, pp 1–32
Wagle A, Desai S, Deo MG (1988) Immunohistochemical localization of ‘Enhancing Factor’, A novel growth modulator, in the mouse small intestines. Cell Biol Int Rep 13:309–311
About this article
Cite this article
Desal, S.J., Mulherkar, R., Wagle, A.S. et al. Ontogeny of enhancing factor in mouse intestines and skin. Histochemistry 96, 371–374 (1991). https://doi.org/10.1007/BF00271359
- Public Health
- Growth Rate
- Small Intestine
- A431 Cell
- High Growth