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The tnpR gene product of TnA is required for transposition immunity

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Summary

A mutant of TnA no longer recognizing immune plasmids has been isolated. The mutation is complemented in trans by a functional tnpR gene. The requirement for wild type tnpR gene product for the establishment of transposition immunity was confirmed by the use of a derivative of transposon Tn3 in which both the tnpA and the tnpR genes are partly deleted. This deleted Tn3 was shown to transpose onto an immune plasmid in the presence of a wild type tnpA gene but not in the presence of both tnpA and tnpR genes.

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References

  1. Achtman M, Willetts N, Clark AJ (1971) Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol 106:529–538

  2. Arthur A, Sherratt D (1979) Dissection of the transposition process: a transposon encoded site-specific recombination system. Mol Gen Genet 175:267–274

  3. Bennett PM, Grinsted J, Richmond MH (1977) Transposition of TnA does not generate deletions. Mol Gen Genet 154:205–211

  4. Bennett PM, Richmond MH (1976) Translocation of a discrete piece of DNA carrying an amp gene between replicons in E. coli. J Bacteriol 126:1–6

  5. Chou J, Lemaux PG, Casadaban MJ, Cohen SN (1979) Transposition protein of Tn3: identification and characterization of an essential repressor-controlled gene product. Nature 282:801–807

  6. Coetzee JN, Datta N, Hedges RW (1972) R factors from Proteus rettgeri. J Gen Microbiol 72:543–552

  7. Cohen SN, Chang ACY, Hsu L (1972) Non-chromosomal antibiotic resistance in bacteria, VII. Genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114

  8. Curtis NAC, Richmond MH, Stanisich V (1973) R-factor mediated resistance to penicillins which does not involve a β-lactamase. J Gen Microbiol 79:163–166

  9. Curtis NAC, Richmond MH (1974) Effect of R-factor-mediated genes on some surface properties of Escherichia coli. Antimicrob Agents Chemother 6:666–671

  10. Datta N, Hedges RW (1972) Trimethoprim resistance conferred by plasmids in Enterobacteriaceae. J Gen Microbiol 72:349–356

  11. Gill R, Heffron F, Dougan G, Falkow S (1973) Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3. J Bacteriol 136:742–756

  12. Gill RE, Heffron F, Falkow S (1979) Identification of the protein encoded by the transposable element Tn3 which is required for its transposition. Nature 282:797–801

  13. Grinsted J, Bennett PM, Higginson S, Richmond MH (1978) Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives. Mol Gen Genet 166:313–320

  14. Grinsted J, Saunders JR, Ingram C, Sykes RB, Richmond MH (1972) Properties of an R-factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol 110:529–537

  15. Heffron F, Bedinger P, Champoux JJ, Falkow S (1977) Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci USA 74:702–706

  16. Heffron F, McCarthy BJ, Ohtsubo H, Ohtsubo E (1979) DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell 18:1153–1163

  17. Humphreys GO, Weston A, Brown MGM, Saunders JR (1979) Plasmid transformation of Escherichia coli. In: Glover SW, Butler LO (eds.) Transformation 1978. Cotswold Press, Oxford, pp 254–279

  18. Jenkins ST, Bennett PM (1978) Effect of mutations in deoxyribonucleic acid repair pathways on the sensitivity of Escherichia coli K-12 strains to nitrofurantoin. J Bacteriol 125:1214–1216

  19. Jobanputra RS, Datta N (1974) Trimethoprim R-factors in enterobacteria from clinical specimens. J Med Microbiol 7:169–177

  20. Kitts P, Symington L, Burke M, Reed R, Sherratt D (1981) Transposon-specified site-specific recombination. Proc Natl Acad Sci USA 78:(in press)

  21. Novick RP, Clowes RC, Cohen SN, Curtiss III R, Datta N, Falkow S (1976) Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev 40:168–189

  22. Richmond MH, Bennett PM, Choi C-L, Brown N, Brunton J, Grinsted J, Wallace L (1980) The genetic basis of the spread of β-lactamase synthesis among plasmid-carrying bacteria. Phil Trans R Soc Lond B 289:349–359

  23. Robinson MK, Bennett PM, Richmond MH (1977) Inhibition of TnA translocation by TnA. J Bacteriol 129:407–414

  24. Rubens C, Heffron F, Falkow S (1976) Transposition of a plasmid deoxyribonucleic acid sequence that mediates ampicillin resistance: independence from host rec functions and orientation of insertion. J Bacteriol 128:425–434

  25. Tu C-PD, Cohen SN (1980) Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Cell 19:151–160

  26. Wallace LJ, Ward JM, Richmond MH (1981) The location of sequences of TnA required for the establishment of transposition immunity. Mol Gen Genet 184:80–86

  27. Ward JM, Grinsted J (1978) Mapping of functions in the R plasmid R388 by examination of deletion mutants generated in vitro. Gene 3:87–95

  28. Witchitz JL, Chabbert YA (1971) Resistance transferable a la gentimicine. I Expression du charactere de resistance. Ann Inst Pasteur Paris 121:733–743

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Communicated by W. Arber

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Wallace, L.J., Ward, J.M. & Richmond, M.H. The tnpR gene product of TnA is required for transposition immunity. Molec. Gen. Genet. 184, 87–91 (1981). https://doi.org/10.1007/BF00271200

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Keywords

  • Gene Product
  • tnpA
  • Immune Plasmid
  • tnpR Gene
  • tnpA Gene