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Adapting liquid spawn Lentinus edodes to oak wood

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Summary

Lentinus edodes (Berk.) Sing. is a commercially important edible mushroom cultivated on oak (Quercus spp.) logs or wood particles. Inoculum (spawn) of this white-rot saprophyte, made by homogenizing cultures grown on a chemically defined liquid medium, dies when transferred to oak wood. This study shows that an aqueous extract of the oak wood is inhibitory to growth. Inoculum produced by homogenizing stationary-grown cultures was particularly sensitive to the extract. Three different methods decreased the sensitivity of inoculum: (1) use of intact (nonhomogenized) cultures as inoculum, (2) use of homogenized cultures produced from shaken rather than stationary cultures as inoculum, or (3) use of cultures grown in the presence of wood extract as inoculum. With the use of shaken cultures adapted to oak wood extract, homogenized-culture inoculum successfully colonized an oak wood medium. Adaptation of the fungus to extracts made from an intended substrate may be an inexpensive way of producing liquid spawn specifically suited for the inoculation of the substrate.

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References

  1. Edwards RL (1978) Cultivation in western countries: growing houses. In: Chang ST, Hayes WA (eds) Academic Press, New York, pp 299–336

  2. Han YH, Yeng WT, Chen LC, Chang S (1981) Physiology and ecology Lentinus edodes (Berk.) Sing. Mushroom Sci 11: 623–658

  3. Ishikawa H, Kawai A, Watanabe H, Oki T (1961) Studies on the biochemical degradation of wood components with special reference on lignin by Lentinus edodes (Berk.) Sing. Rep Tottori Mycol Inst 1: 35–44

  4. Ito T (1978) Cultivation of Lentinus edodes. In: Chang ST, Hayes WA (eds) The biology and cultivation of edible mushrooms. Academic Press, New York pp 461–473

  5. Leatham GF (1982) Cultivation of shiitake, the Japanese forest mushroom, on logs: a potential industry for the United States. Forest Prod J 32: 29–35

  6. Leatham GF (1983) A chemically defined medium for the fruiting of Lentinus edodes. Mycologia 75: 905–908

  7. Leatham GF, Kirk TK (1983) Regulation of ligninolytic activity by nutrient nitrogen in white-rot basidiomycetes. FEMS Microbiol Lett 16: 65–67

  8. San Antonio JP (1981) Cultivation of the shiitake mushroom. HortSci 16: 151–156

  9. Seikel MK, Hostettler FD, Niemann GJ (1971) Phenolics of Quercus rubra wood. Phytochem 10: 2249–2251

  10. Stoller BB (1962) Some practical aspects of making mushroom spawn. Mushroom Sci 5: 170–184

  11. Taguchi H, Yoshida T, Tomita Y, Teramoto S (1968) The effects of agitation on disruption of the mycelial pellets in stirred fermentors. J Ferment Technol 46: 814–822

  12. Wuest PJ (1983) Resources needed to farm the champignon. Mycologia 74: 341–350

  13. Zafar SL (1971) Biochemistry of plant disease resistance: phenolics and the related oxidative systems. Biologia 17: 143–220

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Author information

Correspondence to G. F. Leatham.

Additional information

This article was written and prepared by U.S. Government employees on official time, and it is therefore in the public domain (i.e., it cannot be copyrighted).

Research carried out while T. J. Griffin was a volunteer worker at Forest Products Laboratory

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Leatham, G.F., Griffin, T.J. Adapting liquid spawn Lentinus edodes to oak wood. Appl Microbiol Biotechnol 20, 360–363 (1984). https://doi.org/10.1007/BF00270601

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Keywords

  • Stationary Culture
  • Liquid Medium
  • Aqueous Extract
  • Edible Mushroom
  • Wood Particle