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On the role of the dnaB protein of Escherichia coli in the replication of λ bacteriophage DNA

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Summary

The interaction between the dnaB protein of E. coli and the gene P product of λ bacteriophage was investigated by measuring the cleavage of closed circular phage DNA after infection of two temperature sensitive dnaB mutants, JG28 and To534 groP - B. Cleavage of superhelical DNA from a λπB mutant phage was observed after infection of either strain whereas superhelical DNA from a wild type phage was only cleaved after infection of JG28. When DNA synthesis in infected cells was blocked by incubation at the nonpermissive temperature, no inhibition of superhelical phage DNA cleavage was observed. It is concluded that in conditions where the dnaB protein has lost the capacity to function in nucleotide polymerization, it is capable of interacting with the λ replication gene products to introduce a break in the phage DNA.

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References

  1. D'Ari, R., Jaffe-Brachet, A., Touati-Schwartz, D., Yarmolinsky, B.: A dnaB analog specified by bacteriophage P1. J. Mol. Biol. 94, 341–366 (1975)

  2. Freifelder, D., Kirschner, I.: A phage λ endonuclease controlled by genes O and P. Virology 44, 223–225 (1971)

  3. Georgopolous, C.P., Herskowitz, I.: E. coli mutants blocked in λ DNA synthesis. In: Bacteriophage lambda (A. Hershey, ed.), pp. 553–564. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971

  4. Kogoma, T.: Two types of temperature sensitivity in DNA replication of an Escherichia coli dnaB mutant. J. Mol. Biol. 103, 191–197 (1976)

  5. Lanka, E., Mikolajczyk, M., Schlich, M., Schuster, H.: Association of the prophage P1 ban protein with the dnaB protein of Escherichia coli. J. Biol. Chem. 253, 4746–4753 (1978)

  6. Lanka, E., Schuster, H.: Replication of bacteriophages in Escherichia coli mutants thermosensitive in DNA synthesis. Mol. Gen. Genet. 106, 274–285 (1970)

  7. Lark, K.G., Wechsler, J.A.: DNA replication in dnaB mutants of Escherichia coli: Gene product interaction and synthesis of 4S pieces. J. Mol. Biol. 92, 145–163 (1975)

  8. McMacken, R., Kessler, S., Boyce, R.: Strand breakage of λ DNA supercoils in infected Lysogens. 1. Genetic and biochemical evidence for two types of nicking processes. Virology 66, 356–371 (1975)

  9. Ogawa, T.: Analysis of the dnaB function of Escherichia coli K12 and the dnaB-like function of P1 prophage. J. Mol. Biol. 94, 327–340 (1975)

  10. Pricer, W.B., Jr., Weissbach, A.: The effect of lysogenic induction with mitomycin C on the deoxyribonucleic acid polymerase of Escherichia coli K 12 (λ). J. Biol. Chem. 239, 2607 (1964)

  11. Saito, H., Uchida, H.: Initiation of the DNA replication of bacteriophage λ in Escherichia coli K12. J. Mol. Biol. 113, 1–25 (1977)

  12. Saito, H., Uchida, H.: Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12. Mol. Gen. Genet. 164, 1–8 (1978)

  13. Salzmann, L.A., Weissbach, A.: Studies on the formation of circular λ DNA. Virology 31, 170–177 (1966a)

  14. Salzman, L.A., Weissbach, A.: Formation of intermediates in the replication of phage λ DNA. J. Mol. Biol. 28, 53–70 (1966b)

  15. Shuster, R.C.: The effect of chloramphenicol and rifampicin on the “nicking” and “membrane attachment” of bacteriophage λ DNA. Virology 62, 472–482 (1974)

  16. Shuster, R.C., Weissbach, A.: Genetic mapping of an endonuclease synthesized by bacteriophage lambda. Nature 223, 852–953 (1969)

  17. Skalka, A.M.: DNA replication-bacteriophage lambda. In: Curr. topics microb. and immunol., vol. 78, pp. 201–238. Berlin-Heidelberg-New York: Springer 1977

  18. Thirion, J.P.: Étude cinétique du controlé génétique des différents formes d'ADN de λ apres infection. Ann. Inst. Pasteur 120, 453–465 (1971)

  19. Tomizawa, J.: Functional cooperation of genes O and P. In: Bacteriophage lambda (A. Hershey, ed.) pp. 549–552. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1971

  20. Ueda, K., McMacken, R., Kornberg, A.: dnaB protein of Escherichia coli: purification and role in the replication of ϕX174 DNA. J. Biol. Chem. 253, 261–269 (1978)

  21. Yochem, J., Uchida, H., Sunshine, M., Saito, H., Georgopolous, C.P., Feiss, M.: Genetic analysis of two genes, dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication. Mol. Gen. Genet. 164, 9–14 (1978)

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Correspondence to Robert C. Shuster.

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Communicated by L. Lerman

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Shuster, R.C. On the role of the dnaB protein of Escherichia coli in the replication of λ bacteriophage DNA. Molec. Gen. Genet. 178, 429–435 (1980). https://doi.org/10.1007/BF00270495

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Keywords

  • Polymerization
  • Nucleotide
  • Gene Product
  • Infected Cell
  • Type Phage