Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Mapping of genes involved in the synthesis of spermidine in Escherichia coli

  • 71 Accesses

  • 17 Citations

Summary

The mapping of three genes (speA, speB and metK) involved in the biosynthesis of spermidine is described. They are located between minutes 56 and 57 on the linkage map of Escherichia coli. The clustering of these genes suggests the possibility of an operon for spermidine synthesis. Two other genes, argP, controlling the production of an arginine permease, and can, conferring resistance to canavanine, were found to be located in the same region.

This is a preview of subscription content, log in to check access.

References

  1. Clark, A. J.: Genetic analysis of a “double-male” strain of Escherichia coli K-12. Genetics 48, 105–120 (1963).

  2. Cooper, P. H., Hirshfield, I. N., Maas, W. K.: Map location of arginyl-tRNA synthetase mutations in Escherichia coli K-12. Molec. gen. Genet. 104, 383–390 (1969).

  3. Glansdorff, N.: Topography of cotransducible arginine mutations in Escherichia coli K-12. Genetics 51, 167–179 (1965).

  4. Greene, R. C., Su, C. H., Holloway, C. T.: S-adenosylmethionine synthetase deficient mutants of Escherichia coli K-12 with impaired control of methionine biosynthesis. Biochem. biophys. Res. Commun. 38, 1120–1126 (1970).

  5. Hirshfield, I. N., Rosenfeld, H. J., Leifer, Z., Maas, W. K.: Isolation and characterization of a mutant of Escherichia coli blocked in the synthesis of putrescine. J. Bact. 101, 725–730 (1970).

  6. Hong, J., Ames, B. N.: Localized mutagenesis of any specific small region of the bacterial chromosome. Proc. nat. Acad. Sci. (Wash.) 68, 3158–3162 (1971).

  7. Kadner, R. J., Maas, W. K.: Regulatory gene mutations affecting arginine biosynthesis in Escherichia coli. Molec. gen. Genet. 111, 1–14 (1971).

  8. Lawrence, D. A., Smith, D. A., Rowbury, R. J.: Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to unhibition by analogues of methionine. Genetics 58, 473–492 (1968).

  9. Maas, W. K.: Genetic defects affecting an arginine permease and repression of arginine synthesis in Escherichia coli. Fed. Proc. 23, 1239–1242 (1965).

  10. Maas, W. K., Leifer, Z., Poindexter, J.: Studies with mutants blocked in the synthesis of polyamines. Ann. N.Y. Acad. Sci. 171, 957–967 (1970).

  11. Tabor, H., Tabor, C. W.: Partial separation of two pools of arginine in Escherichia coli; preferential use of exogenous rather than endogenous arginine for the biosynthesis of 1,4-diaminobutane. J. biol. Chem. 244, 6383–6387 (1969).

  12. Taylor, A. L.: Current linkage map of Escherichia coli. Bact. Rev. 34, 155–175 (1970).

Download references

Author information

Additional information

Communicated by E. Witkin

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Maas, W.K. Mapping of genes involved in the synthesis of spermidine in Escherichia coli . Molec. Gen. Genet. 119, 1–9 (1972). https://doi.org/10.1007/BF00270439

Download citation

Keywords

  • Escherichia Coli
  • Arginine
  • Spermidine
  • Canavanine
  • Spermidine Synthesis