In order to clarify the stage of abnormal activation of systemic lupus erythematosus (SLE) B cells, we investigated the cell-cycle phase of SLE B cells by flow-cytometric analysis. This study uses the simultaneous flow-cytometric analysis of cellular DNA content and incorporated bromodeoxyuridine, an analogue of thymidine, as indicators for DNA synthesis to detect activated B cells in peripheral blood of patients with SLE. In active SLE, the percentage of B cells increased in the S and G2M phase and decreased in the G0G1 phase as compared with normal control subjects. In inactive SLE, the percentage of B cells in the S phase also increased. Active SLE B cells did not progress through the cell cycle with the stimulation of anti-IgM plus PMA or SAC plus BSF, although normal B cells transit into S and G2M phase with such stimulation. These data suggest that SLE B cells are already activated and shifted to a matured state and that for this reason the B cells were poorly responsive to mitogen.
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Budman DR, Merchant EB, Steinberg AD, Doff B, Gershwin ME, Lizzio E, Reeves JP (1977) Increased spontaneous activity of antibody-forming cells in the peripheral blood of patients with active SLE. Arthritis Rheum 20:829–833
Ginsburg WW, Finkelman FD, Lipsky PE (1979) Circulating and pokeweed mitogen-induced immunoglobulin-secreting cells in systemic lupus erythematosus. Clin Exp Immunol 35:76–88
Blease MR, Grayson J, Steinberg AD (1980) Increased immunoglobulin-secreting cells in the blood of patients with active systemic lupus erythematosus. Am J Med 69:345–350
Fauci AS, Moutsopoulos HM, (1981) Polyclonally triggered B cells in the peripheral blood and bone marrow of normal individuals and patients with systemic lupus erythematosus and primary Sjögren's syndrome. Arthritis Rheum 24:577–584
Jasin HE, Ziff M (1975) Immunoglobulin synthesis by peripheral blood cells in systemic lupus erythematosus. Arthritis Rheum 18:219–228
Nies KM, Lois JS (1978) Impaired immunoglobulin synthesis by peripheral blood lymphocytes in systemic lupus erythematosus. Arthritis Rheum 21:51–57
Okudaira K, Tanimoto K, Nakamura T, Horiuchi Y (1980) Spontaneously enhanced in vitro immunoglobulin synthesis by B cells in systemic lupus erythematosus. Clin Immunol Immunopathol 16:267–278
Hirose T, Hara M, Kitani A, Hirose W, Norioka K, Kawagoe M, Nakamura H (1985) Abnormal production of and response to B-cell growth factor and B-cell differentiation factor in patients with systemic lupus erythematosus. Scand J Immunol 21:141–150
Tan EM, Cohen AS, Fries JF, Masi AT, Mc Shane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ (1982) The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 258:1271–1277
Schur PH, Sandson J (1968) Immunologic factors and clinical activity in systemic lupus erythematosus. N Engl J Med 278:533–540
Dolbeare F, Gratzner H, Pallavicini MG, Gray JW (1983) Flow cytometric measurement of total DNA content and incorporated bromode-oxyuridine. Proc Natl Acad Sci USA 80:5573–5577
Glinski W, Gerschwin ME, Budman DR, Steinberg AD (1976) Study of lymphocyte subpopulations in normal humans and patients with systemic lupus erythematosus by fractionation of peripheral blood lymphocytes on a discontinuous Ficoll gradient. Clin Exp Immunol 26:228–238
Horwitz DA, Stastny P, Ziff M (1970) Circulating deoxyribonucleic acid synthesizing mononuclear leukocytes. I. Increased numbers of proliferating mononuclear leukocytes in inflammatory disease. J Lab Clin Med 76:391–402
Delbarre F, Le Go A, Kahan A (1975) Hyperbasophilic immunoblasts in circulating blood in chronic inflammatory rheumatic and collagen disease. Ann Rheum Dis 34:422–430
Alarcón-Segovia D, Llorente L, Fishbein E, Diaz-Jouanen E (1982) Abnormalities in the content of nucleic acid of peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Relationship to DNA antibodies. Arthritis Rheum 23:304–317
Klinman DM, Mushinski JF, Honda M, Ishigatsubo Y, Mountz JD, Raveche ES, Steinberg AD (1986) Oncogene expression in autoimmune and normal peripheral blood mononuclear cells. J Exp Med 163:1292–1307
Noronha ABC, Richman DP, Arnason BGW (1980) Detection of in vivo stimulated cerebrospinal-fluid lymphocytes by flow cytometry in patients with multiple sclerosis. N Engl J Med 303:712–717
Suzuki N, Sakane T, Ueda Y, Tsunematsu T (1986) Abnormal B cell function in patients with Behçet disease. Arthritis Rheum 29:212–219
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Hara, M., Kitani, A., Harigai, M. et al. Differential abnormality in cell-cycle stage of peripheral B cells from patients with systemic lupus erythematosus. Rheumatol Int 7, 83–87 (1987). https://doi.org/10.1007/BF00270312
- Cell cycle
- B cells
- Systemic lupus erythematosus