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Continuous culture growth of photoautotrophic cell suspensions from Chenopodium rubrum

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Abstract

The construction and operation of a continuous culture system for the propagation of cell suspensions from Chenopodium rubrum under photoautotrophic conditions has been described. A dilution rate of 0.16/day gave an equilibrium culture density of 1,100,000 cells/ml and a mean doubling time of 150 hours. During continuous culture steady state conditions with respect to nutrient uptake, cell protein and chlorophyll content, starch accumulation, in vitro activities of enzymes related to different metabolic pathways could be established. Photosynthetic CO2 assimilation of steady state cells was about 100 mol CO2/mg chlorophyll x hour. Dark CO2 fixation was 3% of the light values.

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Abbreviations

C. rubrum:

Chenopodium rubrum

DTE:

dithioerythritol

EDTA:

ethylene-diamine-tetraacetic acid

HEPES:

N-2-hydroxy-ethylpiperazine-N-2-ethansulfonic acid

S.E.x:

standard error of the mean

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Hüsemann, W. Continuous culture growth of photoautotrophic cell suspensions from Chenopodium rubrum . Plant Cell Reports 2, 59–62 (1983). https://doi.org/10.1007/BF00270165

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Keywords

  • Chlorophyll
  • Chlorophyll Content
  • Dilution Rate
  • Continuous Culture
  • Culture Density