The construction and operation of a continuous culture system for the propagation of cell suspensions from Chenopodium rubrum under photoautotrophic conditions has been described. A dilution rate of 0.16/day gave an equilibrium culture density of 1,100,000 cells/ml and a mean doubling time of 150 hours. During continuous culture steady state conditions with respect to nutrient uptake, cell protein and chlorophyll content, starch accumulation, in vitro activities of enzymes related to different metabolic pathways could be established. Photosynthetic CO2 assimilation of steady state cells was about 100 mol CO2/mg chlorophyll x hour. Dark CO2 fixation was 3% of the light values.
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- C. rubrum:
standard error of the mean
Bergmeyer HU (1970) Methoden der enzymatischen Analyse, Verlag Chemie Weinheim
Dalton CC (1980) J. exp. Bot. 31: 791–804
Fowler MW (1977) In: Barz W, Reinhard E, Zenk MH (eds.) Plant Tissue Culture and Its Biotechnological Application, Springer Verlag Berlin, Heidelberg, New York
Hüsemann W, Plohr A, Barz W (1979) Protoplasma 100: 101–112
Hüsemann W (1981) Protoplasma 109: 415–431
Hüsemann W (1982) Protoplasma 113: 214–220
Jordan DB, Fletcher JS (1979) Plant Science Letters 17: 95–99
Laetsch WM, Stetler DA (1965) Am. J. Bot. 52: 798–804
Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–479
Peel E (1982) Plant Science Letters 24: 147–155
Warburg O, Geissler AW, Lorenz S (1961) In: Warburg O (ed.) Weiterentwicklung der zellphysiologischen Methoden, Thieme Verlag Stuttgart
Wilson G (1980) In: Fiechter A (ed.) Advances in Biochemical Engineering, Vol. 16, Plant Cell Cultures I, Springer Verlag Berlin, Heidelberg, New York, pp 1–25
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Hüsemann, W. Continuous culture growth of photoautotrophic cell suspensions from Chenopodium rubrum . Plant Cell Reports 2, 59–62 (1983). https://doi.org/10.1007/BF00270165
- Chlorophyll Content
- Dilution Rate
- Continuous Culture
- Culture Density