Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Celery transformation by Agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants

  • 63 Accesses

  • 28 Citations


Transgenic celery plants were obtained following co-cultivation of petiole explants with Agrobacterlum tumefaciens containing pMON200, a cointegrate vector carrying genes for kanamycin resistance and nopaline synthase. Transformants were selected by ability of callus to grow in the presence of 50mg/l kanamycin. Transformation was confirmed either by the presence of nopaline or by Southern blots. Cytological analysis of 14 transformed plants revealed chromosomal aberrations, both in structure and number. Only 20% of the regenerated plants had the normal karyotype. Kanamycin resistance behaved as a monogenic, dominant trait, segregating in a 3:1 ratio in three families derived from plants with normal karyotypes.

This is a preview of subscription content, log in to check access.





2,4-diphenoxyacetic acid


  1. Brower MA and Orton TJ (1986) In: Bajaj PS (ed) Biotechnology in Agriculture and Forestry. Vol. 2: Crops I. Springer-Verlag, Berlin, pp 405–420

  2. Chyi YS, Jorgensen AJ, Goldstein D, Tanksley SD, Loai za-Figueroa F (1986) Mol. Gen. Genet. 204:64–69

  3. Feinberg AP, Vogelstein B (1983) An. Biochem. 132:6–13.

  4. Fischoff DA, Bowdish KS, Periak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean DA, Kusano-Kretzmer K, Mayer EJ, Rochester DE, Rogers SG, Fraley RT. (1987) Bio/technology. 5(8):807–813

  5. Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry JS, Galluppi GR, Goldbert SB, Hoffmann NL, Woo SC (1983) PNAS 80:4803–4807

  6. Fraley TF, Rogers SG, Horsch RB (1986) In: CRC Critical Reviews in Plant Sciences Vol 4 issue 1 pp 1–46

  7. Gamborg OL, Miller RA and Ojima K (1986) Expt. Cell Res. 50:151–158.

  8. Haskell G, Wills AB (1986) Primer of Chromosome Practice, Oliver & Boyd, London

  9. Horsch RB, Fry JE, Hoffmann NL, Eicholtz D, Rogers SG, Fraley RT (1985) Science 227:1229–1231

  10. Marton and Maliga, P (1975) Plant Sci. Lett. 5:577–581

  11. McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Plant Cell Tissue Organ Culture 4:159–169

  12. Otten LA, Schilperoort RA (1978) Biochem Biophys Acta 527:497–500

  13. Williams L and Collin HA (1976) Ann. Bot. 40:32–35.

Download references

Author information

Correspondence to C. F. Quiros.

Additional information

Communicated by L. K. Grill

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Catlin, D., Ochoa, O., McCormick, S. et al. Celery transformation by Agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants. Plant Cell Reports 7, 100–103 (1988). https://doi.org/10.1007/BF00270114

Download citation


  • Transgenic Plant
  • Genetic Analysis
  • Kanamycin
  • Southern Blot
  • Chromosomal Aberration