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Celery transformation by Agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants

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Abstract

Transgenic celery plants were obtained following co-cultivation of petiole explants with Agrobacterlum tumefaciens containing pMON200, a cointegrate vector carrying genes for kanamycin resistance and nopaline synthase. Transformants were selected by ability of callus to grow in the presence of 50mg/l kanamycin. Transformation was confirmed either by the presence of nopaline or by Southern blots. Cytological analysis of 14 transformed plants revealed chromosomal aberrations, both in structure and number. Only 20% of the regenerated plants had the normal karyotype. Kanamycin resistance behaved as a monogenic, dominant trait, segregating in a 3:1 ratio in three families derived from plants with normal karyotypes.

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Abbreviations

KB:

Kilobases

2-4D:

2,4-diphenoxyacetic acid

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Author information

Correspondence to C. F. Quiros.

Additional information

Communicated by L. K. Grill

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Catlin, D., Ochoa, O., McCormick, S. et al. Celery transformation by Agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants. Plant Cell Reports 7, 100–103 (1988). https://doi.org/10.1007/BF00270114

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Keywords

  • Transgenic Plant
  • Genetic Analysis
  • Kanamycin
  • Southern Blot
  • Chromosomal Aberration