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Plant regeneration and protoplast culture of Browollia speciosa

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Abstract

Explants from hypcotyls and cotyledons of Browalia speciosa were shown to regenerate plantlets.

Protoplasts were isolated from etiolated cotyledon material, and, although callus was readily obtained, plantlet regeneration was not observed using numerous hormone regimes.

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Abbreviations

M:

Mannitol

2,4-D:

Dichlorophenoxyacetic acid

NAA:

Naphthalene-acetic acid

BAP:

Benzylaminopurine

MS medium:

Murashige and Skoog (1962) medium

UM medium:

Uchimiya and Murashige (1974) medium

COT:

cotyledon

SH:

shoot

R:

root

References

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Communicated by H. Lörz

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Rybczyński, J.J. Plant regeneration and protoplast culture of Browollia speciosa . Plant Cell Reports 8, 383–386 (1989). https://doi.org/10.1007/BF00270074

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Key Words

  • Browallia speciosa
  • callus induction
  • shoot regeneration
  • plantlets
  • protoplast isolation and culture