Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Phytochrome-mediated responses of cells and protoplasts of green calli obtained from the leaves of a CAM plant

  • 34 Accesses

  • 3 Citations

Abstract

Green callus obtained from leaves of the CAM-inducible plant Kalanchoe blossfeldiana cv. Montezuma has previously been shown to perform C3-type photosynthesis under 16-h days and to shift to crassulacean acid metabolism (CAM) under 9-h days. The utilization of photoperiodic regimes (i.e. night interruptions by 30 min red light) established that CAM induction in the callus was under the control of phytochrome, as shown by measurements of CAM criteria: phosphoenolpyruvate carboxylase activity and malic acid pools. Short-term responsiveness of the callus cells to phytochrome modulations by monochromatic radiations was also established by the rapid changes observed in the diameter of the callus-derived protoplasts. These results provide further evidence that whole plant correlations are not necessary for phytochrome operativity.

This is a preview of subscription content, log in to check access.

Abbreviations

CAM:

crassulacean acid metabolism

PAL:

phenylalanine ammonia lyase (EC 4.3.1.5)

PAR:

photosynthetically active radiations

PEPC:

phosphoenolpyruvate carboxylase (EC 4.1.1. 31)

Rubisco:

ribulose 1,5 bisphosphate carboxylase (EC 4.1.1.39)

References

  1. Alibert G, Brulfert J and Boudet A M (1981) Plant Sci. Lett. 23:341–348.

  2. Blakeley SD, Thomas B, Hall JL and Vince-Prue D (1983) Planta 158: 416–421.

  3. Brulfert J and Güçlü S (1983) Physiol. Vég. 21: 987–995.

  4. Brulfert J, Müller D, Kluge M and Queiroz O (1982) Planta, 154: 326–331.

  5. Brulfert J, Vidal J, Keryer E, Thomas M, Gadal P and Queiroz O (1985) Physiol. Vég. 23: 921–928.

  6. Brulfert J, Mricha A, Sossountzov L and Queiroz O (1987) Plant Cell Environ. 10: 443–449.

  7. Hohorst HJ (1970) In: Bergmeyer H V (ed), Methods in enzymatic analysis, Vol 2, Verlag Chemie, Weinheim, pp. 1544–1548.

  8. Kluge M, Hell R, Pfeffer A and Kramer D (1987) Plant Cell Environ. 10: 451–462.

  9. Lamb CJ and Lawton MA (1983) In: Shropshire W Jr, Mohr H (eds) Encyclopedia of Plant Physiol, Vol 16A Photomorphogenesis, Springer Verlaga, Berlin, pp 213–243.

  10. Pierre JN and Queiroz O (1986) Plant Sc. 45: 179–187.

  11. Queiroz O and Brulfert J (1982) In: Ting IP, Gibbs M (eds) Crassulacean Acid Metabolism, Waverly Press, Baltimore, pp. 208–230.

  12. Sato F, Asada K and Yamada Y (1979) Plant Cell Physiol. 20: 193–200.

  13. Schopfer P (1977) Ann. Rev. Plant Physiol. 28: 223–252.

Download references

Author information

Correspondence to A. Mricha.

Additional information

Communicated by A.M. Boudet

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mricha, A., Brulfert, J., Pierre, J.N. et al. Phytochrome-mediated responses of cells and protoplasts of green calli obtained from the leaves of a CAM plant. Plant Cell Reports 8, 664–666 (1990). https://doi.org/10.1007/BF00269988

Download citation

Keywords

  • Malic Acid
  • Phosphoenolpyruvate
  • Crassulacean Acid Metabolism
  • Phosphoenolpyruvate Carboxylase
  • Callus Cell