Hexaploid wheat plants were easily regenerated from young embryo-derived callus for twelve genotypes tested. After a 2.5 years culture period, however, most of the callus cells lost their ability to regenerate into shoots, but not into roots.
A novel approach was used to regenerate shoots from the long-term suspension cultured cells. In general, instead of selecting embryogenic callus as source material, this approach requires the inoculation of unselected callus into liquid medium followed by removing the free floating cell portion, selecting out non-root forming cell clumps from the root forming primary suspension culture, and growing the putative shoot-competent clumps in liquid medium with reduced auxin concentrations. We have successfully established shoot-competent wheat suspension cultures for cv. ‘Mustang’. High (>80%) frequencies of plant regeneration were observed from plating of 2.5 year suspension cultures. The suspension cultures established by this approach have been utilized to select for heat tolerant variants and will be an ideal source material for protoplast culture and transformation studies. This approach can also be applied to other cereal crops which form roots easily but are unstable in maintaining long term regenerable cultures and which are not easily adaptable to suspension culture.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Christianson, ML (1987) In: Green CE, Somers DA, Hackett WP, Biesborer DD (eds) Plant Tissue and Cell Culture. Proceedings of the VIth International Congress on Plant Tissue and Cell Culture. Alan R. Liss Inc. N.Y. pp. 45–47.
Harris R, Wright M, Byrne M, Vernum J, Brightwell B, Schubert K (1988) Plant Cell Reports 7:337–340.
He DG, Tanner G, Scott KJ (1986) Plant Sci. 45:119–124.
Lazzeri PA, Lorz Horst (1988) Advances in Cell Culture 6:291–325.
Maddock SE (1987) Plant Cell Reports 6:23–26.
Murashige T, Skoog F (1962) Physiol Plant 15:473–497.
Shang XM, Wang WC (1990) Genome (submitted).
Vasil IK, (1988) Biotechnology 6:397–402.
Vasil V, Vasil IK (1984) In: Cell Culture and Somatic Cell Genetics of Plant Vol. 1 ed by Vasil IK Academic Press. Orlando. pp. 152–157.
Wang WC, Beyl CA, Sharma GC (1983) Plant Sci Letter 31:149–156.
Wang WC, Beyl CA, Sharma GC (1988) Cereal Research Comm. 16(1–2):69–76.
Wang WC, Nguyen HT (1989) Plant Cell Report 8:108–111.
Widholm JM (1972) Stain Technology 47:189–194.
Communicated by J.M. Widholm
About this article
Cite this article
Wang, W.C., Nguyen, H.T. A novel approach for efficient plant regeneration from long-term suspension culture of wheat. Plant Cell Reports 8, 639–642 (1990). https://doi.org/10.1007/BF00269982
- Wheat suspension culture