The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-13C] proline was followed by 13C NMR and ninhydrin analyses of suspensions cultured in the presence of 0 or 260 mM NaCl. Uptake of 85 to 92% of the exogenous proline occurred within 72 h in all media. In 10 mM proline and no NaCl, cellular proline reached a maximm of 51.5 μmoles/g FW compared to 1.9 μmoles/g FW in suspensions not grown on proline. In medium containing 260 mM NaCl and proline, cellular proline reached 59–65 μmoles/g FW compared to 30–40 μmoles/g FW in controls grown without proline. The 13C-label in the proline-C1 was either retained in proline or disappeared, presumably released as carbon dioxide, by catabolism through the TCA cycle. Since no metabolite of 13C-proline was detected by NMR, proline was considered to be the molecule which inhibited the suspension culture growth.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Linsmaier and Skoog medium
tricarboxylic acid cycle
nuclear magnetic resonance spectroscopy
- T1 :
spin-lattice relaxation time
Nuclear Overhauser Effect.
Bernt E, Bergmeyer, HU (1965) In: Bergmeyer, HU (ed) Methods of enzymatic analysis, (1st ed.) Academic, NY pp 384–388
Borchett R, Everett GW (1987) Plant Physiol 84:944–949
Chinard FP (1952) J Biol Chem 199:91–95
Daines RJ, Gould AR (1985) J Plant Physiol 119:269–280
Duncan DR, Widholm JM (1987) Plant Physiol 83:703–708
Elthon TE, Stewart CR, (1982) Plant Physiol 70:567–572
Flowers TJ, Troke PF, Yeo AR (1977) Annu Rev Plant Physiol 28:89–121 enzymatic analysis, vol 3 (2nd English ed.) Verlag Chemie Weinheim
Gutmann I, Wahlefeld, WA (1974) In: Bergmeyer, HU (ed) Methods of enzymatic analysis, vol 3 (2nd English ed.) Verlag Chemie Weinheim Academic, San Francisco, pp 1585–1589
Handa S, Handa AK, Hasegawa, PM, Bressan RA (1986) Plant Physiol 80:938–945
Hanson AD, Hitz WD (1982) Annu Rev Plant Physiol 33:163–203
Hooker M, Nabors MW (1977) Z Pflanzenphysiol 84:237–246
Jackson PJ, Unkefer CJ, Doolen JA, Watt K, Robinson NJ (1987) Proc Natl Acad USA 84:6619–6623
Linskens HF, Jackson, JF (1986) Modern methods of plant analysis, New series vol 2, Nuclear Magnetic Resonance. Springer-Verlag Berlin New York
Linsmaier FM, Skoog F (1965) Physiol Plant 8:100–127
Lone MI, Kueh JSH, Wyn Jones RG, Bright SWJ (1987) J Expt Bot 38:477–490
Mahler HR, Cordes EH (1966) Biological Chemistry, Harper and Row, New York
Moftah AE, Michel BE (1987) Plant Physiol 83:238–240
Neeman M, Aviv D, Degani H, Galun E (1985) Plant Physiol 77:374–378
Pandey R, Ganapathy PS, (1985) Plant Sci 40:13–17
Stidham MA, Moreland DE, Siedow JN (1983) Plant Physiol 73:517–520
Troll W, Lindsay J (1955) J Biol Chem 215:655–660
Warren RS, Gould AR (1982) Z Pflanzenphpysiol 107:347–356
Watad AA, Reinhold L, Lerner HR (1983) Plant Physiol 73:624–629
Wuthrich K (1976) NMR in biological research: Peptides and proteins. American Elsevier, New York
Communicated by J. Widholm
About this article
Cite this article
Rodriguez, M.M., Heyser, J.W. Growth inhibition by exogenous proline and its metabolism in saltgrass (Distichlis spicata) suspension cultures. Plant Cell Reports 7, 305–308 (1988). https://doi.org/10.1007/BF00269924
- Carbon Dioxide
- Growth Inhibition
- Suspension Culture