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Growth inhibition by exogenous proline and its metabolism in saltgrass (Distichlis spicata) suspension cultures

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Abstract

The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-13C] proline was followed by 13C NMR and ninhydrin analyses of suspensions cultured in the presence of 0 or 260 mM NaCl. Uptake of 85 to 92% of the exogenous proline occurred within 72 h in all media. In 10 mM proline and no NaCl, cellular proline reached a maximm of 51.5 μmoles/g FW compared to 1.9 μmoles/g FW in suspensions not grown on proline. In medium containing 260 mM NaCl and proline, cellular proline reached 59–65 μmoles/g FW compared to 30–40 μmoles/g FW in controls grown without proline. The 13C-label in the proline-C1 was either retained in proline or disappeared, presumably released as carbon dioxide, by catabolism through the TCA cycle. Since no metabolite of 13C-proline was detected by NMR, proline was considered to be the molecule which inhibited the suspension culture growth.

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Abbreviations

LS:

Linsmaier and Skoog medium

FW:

fresh weight

DW:

dry weight

P5C:

Δ1-pyrroline-5-carboxylate

TCA:

tricarboxylic acid cycle

FID:

free-induction-decay

NMR:

nuclear magnetic resonance spectroscopy

T1 :

spin-lattice relaxation time

NOE:

Nuclear Overhauser Effect.

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Author information

Correspondence to Manuel M. Rodriguez.

Additional information

Communicated by J. Widholm

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Rodriguez, M.M., Heyser, J.W. Growth inhibition by exogenous proline and its metabolism in saltgrass (Distichlis spicata) suspension cultures. Plant Cell Reports 7, 305–308 (1988). https://doi.org/10.1007/BF00269924

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Keywords

  • Dioxide
  • Carbon Dioxide
  • Proline
  • Growth Inhibition
  • Suspension Culture