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Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets

Abstract

Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were found to be quite simple: protoplasts could be cultured in MS, B5 or PGo based media with 0.4 M glucose with the optimum result being produced on KM8p medium. Plating efficiency (P.E) was genotype-dependent with the sugar beet giving better P.E. than the fodder or garden beets used, and higher values being achieved with the use of desalted Driselase for isolation followed by culture on KMBp medium.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

N6 benzylaminopurine

NAA:

naphthaleneacetic acid

P.E.:

plating efficiency

*:

University of Birmingham beet germplasm accession number

References

  1. de Greef W, Jacobs M (1979) Plant Sci Lett 17: 55.

  2. Evans DA, Bravo JE (1984) Handbook of plant cell culture vol.1 Evans DA, Sharp WR, Ammirato PV, Yamada Y eds. Macmillan Publishing Co. New York and London: 124–176.

  3. Evans PK, Cocking EC (1977) Street HE ed. Plant tissue and cell culture, Botanical Monographs, vol. II, Blackwell, Oxford.

  4. Frearson EM, Power JB, and Cocking EC (1973) Dev Biol 33: 130.

  5. Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151.

  6. Kao KN, Michayluk MR (1975) Planta 126: 105.

  7. Murashige T, Skoog F (1962) Physiol Plant 15: 473.

  8. Patnaik G, Wilson D, Cocking EC (1981) Z Pflanzenphysiol 102: 199–205.

  9. Santos A, Dos VP, Cocking EC, Davey MR (1980) Z Pflanzenphysiol 99: 261–270.

  10. Saunders JW, Daub ME (1984) Plant Sci Lett 34: 219–223.

  11. Smith MAL, Palta J, McCown BH (1984) Plant Sci Lett 33: 249.

  12. Smolenskaya IN, Raldugina GN (1981) Soviet Plant Physiol 28: 752–58.

  13. Szabados L, Gaggero C (1983) Proc 6th Int Protoplast Symp Experientia suppl 45: 38.

  14. Szabados L, Gaggero C (1985) Plant Cell Reports 4: 195–8.

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Author information

Correspondence to B. V. Ford-Lloyd.

Additional information

Communicated by B. L. Miflin

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Bhat, S.R., Ford-Lloyd, B.V. & Callow, J.A. Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets. Plant Cell Reports 4, 348–350 (1985). https://doi.org/10.1007/BF00269896

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Keywords

  • Glucose
  • Sugar
  • Cellulase
  • Suspension Culture
  • Sugar Beet