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Plant regeneration from mesophyll protoplasts in commercial potato cultivars (Primura, Kennebec, Spunta, Desirée)

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Abstract

In view of an evaluation of the relative efficiency of different in vitro systems for mutant induction and isolation in potato, a procedure of plant regeneration from protoplasts of some potato (Solanum tuberosum L.) cultivars was developed. Four cultivars (Primura, Kennebec, Spunta, Desirée) were used for isolation, culture and regeneration of leaf mesophyll protoplasts.

These lines were chosen because of their economic importance in Italy and in the case of cv. Desirée for the presence of markers useful in the morphological characterization of regenerants.

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Abbreviations

MS :

Murashige and Skoog's medium

BAP :

6-benzylaminopurine

2,4 D :

2,4-dichlorophenoxyacetic acid

NAA :

naphthaleneacetic acid

GA 3 :

gibberellic acid

ZT :

zeatin

References

  1. Bokelmann G.S. and Roest S. (1983). Z. Pflanzenphysiol. 109: 259–265.

  2. Broertjes, C. and A.M. Van Harten (1978) in: Application of mutation breeding in the improvement of vegetatively propagated crops: an interpretative literature review. Elsevier Sci. Publ. Co.

  3. Harten A.M. Van (1978) Agric. Res. Rep. 873.DUPOC Wageningen 132 p.

  4. Harten A.M. Van, and Bouter H. (1973) Euphytica 22: 1–7.

  5. Karp A., Nelson R.S., Thomas E., Bright S.W.J. (1982) Theor. Appl. Genet. 63: 265–272.

  6. Larkin, P.J., Scowcroft W.R. (1981): Theor. Appl. Genet. 60: 197–214.

  7. Lörz H., Larkin P.J., Thomson J. and Scowcroft W.R. (1983) Plant Cell Tissue Organ Culture 2: 217–226.

  8. Murashige T. and Skoog F. (1962). Physiol. Plant. 15: 437–497.

  9. Roest S. and G.S. Bokelmann (1983) in: I. Potrykus, C. T. Harms, A. Hinnen, R. Hutter, P.J. King and R. D. Shillito (Eds.), PROTOPLASTS 1983 Post Proc., Birkhauser Verlag Basel - Boston -Stuttgart p. 282–283

  10. Shepard J.F. and Totten R.. (1977) Plant Physiol. 60: 313–316.

  11. Shepard J.F. (1980) In: Genetic Improvement of Crops: Emergent Techniques 185–189, Minneapolis, Univ. of Minnesota Press.

  12. Sonnino A., Ancora G. and Locardi C. (1985) In: International Symposium on Nuclear Techniques and in-vitro culture for plant improvement, IAEA, Wien; p. 385–394.

  13. Wenzel, G. (1980) In: Advance in Protoplast Research 327–340 Oxford: Pergamon Press.

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Author information

Correspondence to G. Ancora.

Additional information

Communicated by H. Lörz

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Tavazza, R., Ancora, G. Plant regeneration from mesophyll protoplasts in commercial potato cultivars (Primura, Kennebec, Spunta, Desirée). Plant Cell Reports 5, 243–246 (1986). https://doi.org/10.1007/BF00269812

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Keywords

  • Plant Regeneration
  • Economic Importance
  • Solanum Tuberosum
  • Relative Efficiency
  • Potato Cultivar