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Plant Cell Reports

, Volume 6, Issue 1, pp 50–54 | Cite as

Marker proteins for embryogenic differentiation patterns in pea callus

  • Susanne Stirn
  • Hans-Jörg Jacobsen
Article

Abstract

Polypeptide pattern alterations during somatic embryogenesis were investigated using callus cultures of two Pisum sativum genotypes. Both genotypes show the formation of two different callus lines from the same explant after six to eight weeks in culture: a nodular yellowish callus line, which forms somatic embryoids in suspension cultures (e+) and a white compact callus line with no regenerative capacity (e). The cytosol proteins of the two different callus lines were separated in a semi-preparative two-dimensional system and the polypeptide patterns were compared. Two protein bands were found (P1: Mr=45000 D, pI=7.0–7.1; P2: Mr=7000 D, pI=<4.5), which were characteristic of the putatively embryogenic (e+) callus line in all tissues investigated (two genotypes × two explant sources). These proteins found in nodular (e+) pea cultures are very similar to two proteins found in Daucus carota suspension cultures preceding the formation of somatic embryos.

Keywords

Cytosol Somatic Embryo Somatic Embryogenesis Suspension Culture Callus Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BA

6-benzyl-aminopurine

Bistris

2-(bis(2-hydroxyethyl)imino)-2-(hydroxymethyl)-1.3-propanediol

PAGE

polyacrylamide gel electrophoresis

pI

isoelectric point

TCA

trichloroacetic acid

Tris

tris(hydroxymethyl)-aminomethane

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Susanne Stirn
    • 1
  • Hans-Jörg Jacobsen
    • 1
  1. 1.Institut für Genetik der Universität BonnBonn 1Federal Republic of Germany

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