Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Tissue culture propagation of Mentha spp. by the use of axillary buds

Abstract

A method is described for rapid multiplication from axillary buds of six Mentha species. Nodal segments from one-year old plants were grown on Murashige and Skoog medium (BMS), supplemented with BAP (1.0; 2.0 mg/l) and KIN (1.0 mg/l) and kept at 28 ± 1°C under fluorescent light for 30 days. After this period, several shoots (15–20 shoots per explant) with roots were produced which were placed in soil for further growth.

This is a preview of subscription content, log in to check access.

References

  1. Bersaghi C (1945) Cultura da Menta. Secretaria de Agricultura, Indústria e Comércio do Estado de São Paulo, 32p.

  2. Chomchalow N, Sahavacharin O (1981) In: Rao AN (ed) The role of tissue culture in the development of Medicinal Plants and Spices, Proc. Symp on tissue culture of Economicaly Important Plants, Singapore. pp. 162–166.

  3. Lin NL, Staba EJ (1961) Lloydia 24:139.

  4. Misawa M (1977) In: Barz W, Reinhard E, Zenk MH (eds) Plant Tissue Culture and its Bio-technological Application, Springer-Verlag, Berlin Heidelberg, New York, pp. 17–26.

  5. Murashige T (1977) Hort Sci 12: 127–130.

  6. Murashige T, Skoog F (1962) Physiol Plant 15: 473–493.

  7. Secretaria do Estado da Agricultura do Paraná. Departamento de Economia Rural (1978) Mentha (Mentha arvensis L.) Curitiba, 39p.

  8. Sugisawa H, Ohnishi Y (1976) Agr Biol Chem 40: 231–232.

  9. Tabata M (1977) In: Barz W, Reinhard E, Zenk MH (eds) Plant Tissue Culture and its Bio-technological Application, Springer-Verlag. Berlin Heidelberg. New York, pp. 3–16.

  10. Wang CJ, Staba EJ (1963) J Pharm Sci 52: 1058–1062.

  11. Whithers LA (1980) Tissue Culture Storage for genetic conservation IBPGR tech Rpt, FAO, Rome.

Download references

Author information

Correspondence to E. L. Rech.

Additional information

Communicated by I.K. Vasil

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rech, E.L., Pires, M.J.P. Tissue culture propagation of Mentha spp. by the use of axillary buds. Plant Cell Reports 5, 17–18 (1986). https://doi.org/10.1007/BF00269708

Download citation

Keywords

  • Tissue Culture
  • Fluorescent Light
  • Rapid Multiplication
  • Nodal Segment
  • Skoog Medium