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Restriction endonuclease cleavage map of pKM101: Relationship to parental plasmid R46


A detailed restriction endonuclease cleavage map of the plasmid pKM101 has been constructed. pKM101 plasmids containing individual Tn5 insertions were used to facilitate the ordering of restriction fragments generated by enzymes cleaving pKM101 at multiple sites. By restriction enzyme analysis, pKM101 (35.4 kilobases) appears to have arisen from its clinically-isolated parent by deletion of a single DNA region which codes for three of the four drug resistances carried by R46.

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  1. Bradley DE (1979) Morphology of pili determined by the N incompatibility group plasmid N3 and interaction with bacteriophage PR4 and IKe. Plasmid 2:632–636

  2. Datta N, Kontomichalou P (1965) Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature (London) 208:239–241

  3. Drabble WT, Stocker BAD (1968) R (transmissible drug-resistance) factors in S. typhimurium: pattern of transduction by phage P22 and ultraviolet protection effect. J Gen Microbiol 53:109–123

  4. Grindley NDF, Humphreys GO, Anderson ES (1973) Molecular studies of R factor incompatibility groups. J Bacteriol 115:387–398

  5. Jorgensen RA, Rothstein SJ, Reznikoff WS (1979) A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet 177:65–72

  6. Khatoon H, Iyer RV, Iyer VN (1972) A new filamentous bacteriophage with sex-factor specificity. Virology 48:145–155

  7. Lackey D, Walker GC, Keng T, Linn S (1977) Characterization of an endonuclease associated with the drug resistance plasmid pKM101. J Bacteriol 131:583–588

  8. McCann J, Choi E, Yamasaki E, Ames BN (1975a) Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci USA 72:5135–5139

  9. McCann J, Spingarn NE, Kobori J, Ames BN (1975b) Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci USA 72:979–983

  10. Meagher RB, Shepard RJ, Boyer HW (1977) The structure of cauliflower mosaic virus. I. A restriction endonuclease map of cauliflower mosaic virus DNA. Virology 80:362–375

  11. Mortelmans KE, Stocker BAD (1976) Ultraviolet light protection, enhancement of ultraviolet mutagenesis, and mutator effect of plasmid R46 in Salmonella typhimurium. J Bacteriol 128:271–282

  12. Mortelmans K, Stocker BAD (1979) Segregation of the mutator property of plasmid R46 from its ultraviolet-protecting property. Mol Gen Genet 167:317–328

  13. Murray K, Murray NE (1975) Phage lambda receptor chromosomes for DNA fragments made with restriction enzyme III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol 98:551–564

  14. Pinney RJ, Smith JT (1974) Fertility Inhibition of an N group R factor by a group X R factor, R6K. J Gen Microbiol 82:415–418

  15. Shanabruch WG, Walker GC (1980) Localization of the plasmid (pKM101) gene(s) involved in recA + lexA+-dependent mutagenesis. Mol Gen Genet 179:289–297

  16. Shapiro JR (1977) Bacterial Plasmids. In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids, and episomes. Cold Springer Harbor Laboratory, Cold Springer Harbor, N.Y., pp 601–670

  17. Summers AO, Jacoby GA (1979) Plasmid-determined resistance to tellurium compounds. J Bacteriol 129:276–281

  18. Walker GC (1977) Plasmid (pKM101)-mediated enhancement of repair and mutagenesis: dependence on chromosomal genes in Escherichia coli K-12. Mol Gen Genet 152:93–103

  19. Walker GC (1978) Isolation and characterization of mutants of the plasmid pKM101 deficient in their ability to enhance mutagenesis and repair. J Bacteriol 133:1203–1211

  20. Walker GC (1979a) Mutagenesis and repair enhancing activities associated with the plasmid pKM101. Cold Spring Harbor Symp Quant Biol 43:893–896

  21. Walker GC (1979b) Theory and design of short term bacterial tests for mutagenesis. In: McElheny VK, Abrahamson S (eds). Assessing chemical mutagens: The risk to humans. Cold Springer Harbor Laboratory, Cold Springer Harbor, N.Y., pp 63–80

  22. Walker GC, Dobson PP (1979) Mutagenesis and repair deficiencies of Escherichia coli umuC mutants are suppressed by the plasmid pKM101. Mol Gen Genet 172:17–24

  23. Walker GC (1980) Molecular principles underlying the Ames Salmonella/microsome test: elements and design of short term mutagenesis tests. In: Kolber A (ed) In vitro toxicity testing of environmental agents. Elsevier Corp., Amsterdam, New York (in press)

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Correspondence to Graham C. Walker.

Additional information

Communicated by G. O'Donovan

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Langer, P.J., Walker, G.C. Restriction endonuclease cleavage map of pKM101: Relationship to parental plasmid R46. Molec. Gen. Genet. 182, 268–272 (1981). https://doi.org/10.1007/BF00269669

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  • Enzyme
  • Restriction Endonuclease
  • Drug Resistance
  • Restriction Fragment
  • Multiple Site