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Lectin levels in tissues of cultured immature wheat embryos

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Abstract

Levels of wheat germ agglutinin have been determined by radioimmunoassay in tissues of immature wheat embryos cultured under different conditions in order to determine the suitability of the lectin as a marker for somatic embryogenesis. Embryos cultured on media favouring continued embryo development accumulated lectin in a similar manner to zygotic embryos in planta unless precocious germination occurred. Embryos cultured on media containing 2,4-D produced callus, and some of this developed somatic embryos. Both embryogenic and non-embryogenic callus contained WGA, that in non-embryogenic callus possibly arising from developmentally arrested root primordia.

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Abbreviations

ABA:

abscisic acid

dpa:

days post anthesis

PBS:

phosphate buffered saline, (10 mM KH2PO4 K2HPO4, 145 mM NaCl, pH 7.4)

RIA:

radioimmunoassay

WGA:

wheat germ agglutinin

2,4-D:

2,4-dichlorophenoxyacetic acid

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Author information

Correspondence to P. C. Morris.

Additional information

Communicated by I. Potrykus

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Morris, P.C., Maddock, S.E., Jones, M.G.K. et al. Lectin levels in tissues of cultured immature wheat embryos. Plant Cell Reports 5, 460–463 (1986). https://doi.org/10.1007/BF00269642

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Keywords

  • Somatic Embryo
  • Somatic Embryogenesis
  • Embryo Development
  • Zygotic Embryo
  • Wheat Germ