Levels of wheat germ agglutinin have been determined by radioimmunoassay in tissues of immature wheat embryos cultured under different conditions in order to determine the suitability of the lectin as a marker for somatic embryogenesis. Embryos cultured on media favouring continued embryo development accumulated lectin in a similar manner to zygotic embryos in planta unless precocious germination occurred. Embryos cultured on media containing 2,4-D produced callus, and some of this developed somatic embryos. Both embryogenic and non-embryogenic callus contained WGA, that in non-embryogenic callus possibly arising from developmentally arrested root primordia.
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days post anthesis
phosphate buffered saline, (10 mM KH2PO4 K2HPO4, 145 mM NaCl, pH 7.4)
wheat germ agglutinin
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Communicated by I. Potrykus
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Morris, P.C., Maddock, S.E., Jones, M.G.K. et al. Lectin levels in tissues of cultured immature wheat embryos. Plant Cell Reports 5, 460–463 (1986). https://doi.org/10.1007/BF00269642
- Somatic Embryo
- Somatic Embryogenesis
- Embryo Development
- Zygotic Embryo
- Wheat Germ