Protoplasts from Olea europaea L. have been compared in terms of their yield, viability, cell division and callus differentiation. Viable protoplasts were isolated from in vitro cultured leaves and cotyledons by an overnight incubation in an enzyme solution containing 1–1.5% driselase and 0.5M sucrose. This method allowed high yield of purified protoplasts, which floated and formed a dark green band at the meniscus, after centrifugation. Purified protoplasts were diluted to 3×104 protoplasts·ml−1 in culture medium. After cell wall regeneration, protoplasts gradually increased their volumes under appropriate conditions. The first divisions occurred during the second week in culture. Division efficiency ranged from 5.2 to 9.8% after 20 days in culture. Two weeks later visible microcolonies developed only from cotyledon protoplasts. After 6 weeks in culture, the microcalli were transferred to a solidified culture medium with 0.6% agarose, which induced active callus growth.
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olive proliferation medium, Rugini 1984
OM for the germination of olive embryos
for root induction
Bourgin and Nitsch medium 1967
naphthalene acetic acid
Ahuja MR (1984). Silvae Genet. 33: 37–39
Bourgin JP, Nitsch JP (1967). Ann. Physiol. Vég. 9:377–382
Cañas LA, Carramolino L, Vicente M (1987). Plant Sci. 50:85–90
Cañas LA (1985). Abstracts of the First Internat.Congress on Plant Molecular Biology. Galau Ed.Athens, USA:102
David H, Jarlet E, David A (1984). Physiol. Plant. 61:477–482
Dorion N, Danthu P, Bigot C (1986). Abstracts of the 4th Internat.Congress of Plant Tissue and Cell Culture. Minnesota, USA:43.
Huhtinen O, Honkanen J, Simola K (1982). Plant Sci. Lett. 28:3–9
Kirby EG (1982). Physiol. Plant. 56: 114–117
Ochatt SJ, Caso SH (1986). J. Plant Physiol. 122:243–249
Oka S, Ohyama K (1985). J. Plant Physiol. 119:455–460
Patel KR, Shekhawat NS, Berlyn GP, Thorpe TA (1984). Plant Cell Tissue Organ Cult. 3: 85–90
Rao P, Ozias-Akins P (1985). Protoplasma 124:80–86
Rugini E (1986). In: Bajaj Ed. Biotechnology in Agriculture and Forestry, vol.I. Spring-Verlag, Berlin pp 253–267
Smith MA, Mac Cown BN (1983). Plant Sci. Lett. 28:149–156
Sticklen MB, Lineberger D, Domir SC (1985). Plant Sci. 41: 117–120
Tremblay FM, Power B, Lalonde M (1985). Plant Sci. 41:211–216
Vardy A, Spiegel-Roy P, Galun E (1982). Theor. Appl. Genet. 62: 171–176
Wang KJ, Zhang PF, Ni DX, Zhu XZ, Yang WQ, Bao ZH (1979). Acta Bot. Sinica. 21: 225–230
Widholm JM (1972). Stain Tech. 47: 189–194
Wu SC, Kuniyuki AH (1985). Plant Sci. 41: 55–60
Communicated by A. M. Boudet
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Cañas, L.A., Wyssmann, A.M. & Benbadis, M.C. Isolation, culture and division of olive (Olea europaea L.) protoplasts. Plant Cell Reports 6, 369–371 (1987). https://doi.org/10.1007/BF00269563
- Cell Wall
- Cell Division
- Solidify Culture Medium