Molecular and General Genetics MGG

, Volume 126, Issue 4, pp 319–324 | Cite as

Evidence that pretreatment of Escherichia coli cells with N-methyl-N′-nitro-nitrosoguanidine enhances mutability of subsequently infecting phage λ

  • Sohei Kondo
  • Haruko Ichikawa


The frequency of mutations in λ is significantly higher for host cells of a rec+ or recA- strain pretreated with N-methyl-N′-nitro-N-nitrosoguanidine (NG) than for untreated control cells. Direct NG treatment of λDNA-injected host cells results in about tenfold higher mutation frequency in λ than NG treatment of host cells alone. Since mutability of λ is enhanced by UV preirradiation of host cells in the rec+ strain but not in the recA- strain, indirect NG mutagenesis is different in mechanism from indirect UV mutagenesis. It is concluded that NG mutagenesis in λ consists of at least two processes: induction of rec+-independent mutagenic activity in the host bacterium and production of rec+-independent mutational damage to intracellular λDNA.


Escherichia Coli Control Cell Host Cell Untreated Control Mutation Frequency 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Sohei Kondo
    • 1
  • Haruko Ichikawa
    • 1
  1. 1.Department of Fundamental Radiology, Faculty of MedicineOsaka UniversityOsakaJapan

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