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Resistance to freezing in liquid nitrogen of carnation (Dianthus caryophyllus L. var Eolo) apical and axillary shoot tips excised from different aged in vitro plantlets

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Abstract

The ability of shoot tips from carnation (Dianthus caryophyllus L., var. Eolo) cultured in vitro to develop resistance to freezing in liquid nitrogen depends on the physiological state of the cell material and the pretreatment conditions. Regrowth rates close to 100% have been obtained with apical shoot tips isolated from 2 month-old stems, precultured on medium supplemented with sucrose (0.75M) and treated with dimethylsulfoxide (5% or more). Resistance of axillary shoot tips decreased progressively as a funtion of their distance from the apical shoot tip. During the development of the stem from axillary buds (obtained by cutting), progressive increases in the regrowth rate of frozen apices were noted, from 30% before cutting (axillary buds) to 98% after 3 weeks of culture.

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Abbreviations

DMSO:

dimethylsulfoxide

LN:

liquid nitrogen

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Author information

Correspondence to J. Dereuddre.

Additional information

Communicated by A. M. Boudet

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Dereuddre, J., Fabre, J. & Bassaglia, C. Resistance to freezing in liquid nitrogen of carnation (Dianthus caryophyllus L. var Eolo) apical and axillary shoot tips excised from different aged in vitro plantlets. Plant Cell Reports 7, 170–173 (1988). https://doi.org/10.1007/BF00269315

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Key words

  • Dianthus caryophyllus
  • carnation
  • apical and axillary shoot tips
  • cryopreservation