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Growth of cucumber cells in media with lactose or milk whey as carbon source

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Abstract

Lactose utilisation by cucumber cell suspension cultures starts only after a long lag phase and is accompanied by an increase of an extracellular lactosespecific β-galactosidase activity. Supplementing the lactose medium with sucrose shortens the lag phase.

Milk whey permeate seems to contain a factor(s) which inhibits lactose utilisation. After supplementing the medium with sucrose or its hydrolysis products, growth and substrate utilisation is as efficient as in Murashige and Skoog medium. Galactose also induces growth, but growth and substrate utilisation are slower. In whey medium, supplemented with sucrose, the extracellular β-galactosidase activity again accompanies growth induction.

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Abbreviations

MS:

Murashige and Skoog medium

MW:

milk whey medium

NAD:

nicotinamide-adenine dinucleotide

ONPG:

o-nitrophenyl-β-D-galactopyranoside

PNPG:

p-nitrophenyl-β-D-galactopyranoside

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Author information

Correspondence to Alfons Callebaut.

Additional information

Communicated by H. Lörz

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Callebaut, A., Motte, J. Growth of cucumber cells in media with lactose or milk whey as carbon source. Plant Cell Reports 7, 162–165 (1988). https://doi.org/10.1007/BF00269313

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Keywords

  • Sucrose
  • Carbon Source
  • Cell Suspension
  • Lactose
  • Galactose