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Medium components for shoot cultures of chlorophyll-deficient mutants of petunia inflata

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Abstract

Selected medium components were tested for 30 day growth promotion of shoot tips of Petunia inflata wild type, a cytoplasmic and a nuclear inherited chlorophyll-deficient mutant. Experiments were conducted independently with iron, sucrose, thiamine-HCl, indole-3-acetic acid (IAA), Kinetin (K), 6-benzylaminopurine (BA), coconut milk (CM), casein hydrolysate (CH), and plant extract (PE) an aqueous leaf extract, added to modified Murashige and Skoog (MS) salts and vitamins medium, and pH between 4.0 and 7.0 was also compared. The optimum concentrations of all test components were used to formulate revised MS media especially designed for in vitro shoot growth of chlorophyll-deficient petunia mutants. The optimum medium for the nuclear albino was: MS salts+1.5 mg/l thiamine-HCl+100 mg/l myo-inositol+3.5% sucrose+1% PE+5.0 mg/l IAA+0.3 mg/l K at pH 6.0; for the wild type: MS salts+0.6 mg/l thiamine-HCl+100 mg/l myo-inositol+4% sucrose+1.0 mg/l IAA+0.3 mg/l K at pH 5.0 and for the cytoplasmic albino: MS salts+0.4 mg/l thiamine-HCl+100 mg/l myo-inositol+4% sucrose+20% CM+3% PE+1.0 mg/l K at pH 5.0. On the revised MS media a 3-, 4- and 5- fold increase in 30 day plant fresh weight occurred for the nuclear, wild type and cytoplasmic chlorophyll-deficient plants, respectively.

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Abbreviations

BA:

6-benzylaminopurine

CA:

cytoplasmic albino

CH:

casein hydrolysate

CM:

coconut milk

IAA:

indole-3-acetic acid

K:

Kinetin

MS:

Murashige and Skoog

NA:

nuclear albino

PE:

plant extract

WT:

wild type

References

  1. Brown, DC, Leung DWM, Thorpe TA (1979) Physiol. Plant. 46:36–41

  2. Coffin R, Taper CD, Chong C (1976) Can. J. Bot. 54:547–551

  3. Gamborg OL, Miller R, Ojima K (1968) Exp. Cell Res. 50:151–158

  4. Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) In Vitro 12:473–478

  5. Linsmaier E, Skoog F (1965) Physiol. Plant. 18:100–127

  6. Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497

  7. Romberger JA, Tabor CA (1971) Amer. J. Bot. 58:131–140

  8. Schaeffer GW, Menser Jr. MA (1975) Crop Science 15:728–730

  9. Schenk RU, Hildebrandt AC (1972) Can. J. Bot. 50:199–204

  10. Schieder O, Vasil IK (1980) In: Vasil IK (ed) Perspectives in plant cell and tissue culture, Suppl. 11B, Academic Press, New York, pp 21–42

  11. Steward FC, Caplin SM (1952) Ann. Bot. 16:491–504

  12. Tulecke W, Weinstein LH, Rutner A, Lavrencot Jr. HJ (1961) Boyce Thompson Institute for Plant Res. 21:115–128

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Michigan Agricultural Experiment Station Journal Article No. 10346.

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Borkird, C., Sink, K.C. Medium components for shoot cultures of chlorophyll-deficient mutants of petunia inflata . Plant Cell Reports 2, 1–4 (1983). https://doi.org/10.1007/BF00269222

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Keywords

  • Plant Extract
  • Kinetin
  • Medium Component
  • Casein Hydrolysate
  • Shoot Culture