A procedure for prolonged cryogenic storage of periwinkle cell cultures is described. Cells derived from periwinkle, Catharanthus roseus (L.) G. Don, and subcultured as suspension in 1-B5C nutrient medium have been frozen, stored in liquid nitrogen (−196°C) for 11 weeks, thawed and recultured. Maximal survival was achieved when 3–4 day-old cells precultured for 24 h in nutrient medium with 5% DMSO were frozen at slow cooling rates of 0.5 or 1°C/min prior to storage in liquid nitrogen. The only loss in viability of cells occurred subsequent to treatment with DMSO. Abbreviations: DMSO, dimethylsulfoxide; 2,4-D, 2,4-dichlorophenoxyacetic acid; TTC, triphenyltetrazolium chloride.
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Kartha, K.K., Leung, N.L., Gaudet-LaPrairie, P. et al. Cryopreservation of periwinkle, Catharanthus roseus cells cultured in vitro. Plant Cell Reports 1, 135–138 (1982). https://doi.org/10.1007/BF00269181
- Catharanthus roseus
- cell cultures
- cryogenic storage