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Cryopreservation of periwinkle, Catharanthus roseus cells cultured in vitro

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A procedure for prolonged cryogenic storage of periwinkle cell cultures is described. Cells derived from periwinkle, Catharanthus roseus (L.) G. Don, and subcultured as suspension in 1-B5C nutrient medium have been frozen, stored in liquid nitrogen (−196°C) for 11 weeks, thawed and recultured. Maximal survival was achieved when 3–4 day-old cells precultured for 24 h in nutrient medium with 5% DMSO were frozen at slow cooling rates of 0.5 or 1°C/min prior to storage in liquid nitrogen. The only loss in viability of cells occurred subsequent to treatment with DMSO. Abbreviations: DMSO, dimethylsulfoxide; 2,4-D, 2,4-dichlorophenoxyacetic acid; TTC, triphenyltetrazolium chloride.

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  1. Barz W, Ellis BE (1981) Ber.Deutsch.Bot.Ges. 94: 1–26 Constabel F (1981) Proc. VIth Intern. Ferment. Symp. London/Ont. July 1980

  2. Dhoot GK, Henshaw GG (1977) Ann.Bot. 41: 943–949

  3. Dougall DK, Whitten GH (1980) Planta Med. Suppl. 129–135

  4. Gamborg OL, Miller RA, Ojima K (1968) Exp.Cell Res. 58: 151–158

  5. Gamborg OL, Wetter LR (1975) In: Gamborg OL, Wetter LR (eds) Plant tissue culture methods, National Research Council of Canada, Saskatoon, p 96

  6. Haskins RH, Kartha KK (1980) Can.J.Bot. 58: 833–840

  7. Kartha KK (1981) In: Thorpe TA (ed) Plant tissue culture methods and applications, Academic Press, Inc, New York, pp 181–211

  8. Kurz WGW, Chatson KB, Constabel F, Kutney JP, Choi LSL, Kolodziejezyk P, Sleigh SK, Stuart KL, Worth BR (1980) Phytochemistry 19: 2583–2587

  9. Kutney JP, Choi LSL, Kolodiejezyk P, Sleigh SK, Stuart KL, Worth BR, Kurz WGW, Chatson KB, Constabel F (1980) Heterocycles 14: 765–768

  10. Nag KK, Street HE (1973) Nature 245: 270–272

  11. Steponkus PL, Lanphear FO (1967) Plant Physiol. 42: 1423–1426

  12. Ulrich JM, Finkle BJ, Moore PH, Ginoza H (1979) Cryobiology 16: 550–556

  13. Withers LA, Street HE (1977) In: Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its biotechnical application, Springer-Verlag, Berlin-Heidelberg-New York, pp 226–244

  14. Zenk MH (1978) In: Thorpe TA (ed) Frontiers of plant tissue culture 1978, Int.Assoc.Plant Tissue Cult. Univ.of Calgary, pp 1–14

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NRCC No. 20082

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Kartha, K.K., Leung, N.L., Gaudet-LaPrairie, P. et al. Cryopreservation of periwinkle, Catharanthus roseus cells cultured in vitro. Plant Cell Reports 1, 135–138 (1982).

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Key Words

  • Catharanthus roseus
  • periwinkle
  • cell cultures
  • DMSO
  • cryopreservation
  • cryogenic storage