The extent of phenotypic reversal by 5-fluorouracil of an amber mutation of the phage T4D increases at high pH. The magnitude of the increase depends strongly on the composition of nutritive medium.
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Champe, S. P., Benzer, S.: Reversal of mutant phenotypes by 5-fluorouracil, an approach to nucleotide sequences in messenger RNA. Proc. nat. Acad. Sci. (Wash.) 48, 532–546 (1962).
Edlin, G.: Gene regulation during bacteriophage T4 development. I. Phenotypic reversion of T4 amber mutants by 5-fluorouracil. J. molec. Biol. 12, 363–374 (1965).
Garen, A., Siddiqi, O.: Suppression of mutations in the alcaline phosphatase structural cistron of E. coli. Proc. nat. Acad. Sci. (Wash.) 48, 1121–1127 (1962).
Kaplan, S., Stretton, A. O. W., Brenner, S.: Amber suppressors: efficiency of chain propagation and suppressor specific amino acids. J. molec. Biol. 14, 528–533 (1965).
Kashket, E. R., Wong, P. T.: The intracellular pH of Escherichia coli. Biochim. biophys. Acta (Amst.) 193, 212–214 (1969).
Putnam, F.W., Kozloff, L.M., Neil, J.C.: Biochemical studies of virus reproduction. I. Purification and properties of E. coli bacteriophage T6. J. biol. Chem. 179, 303–323 (1949).
Rosen, B., Rothman, F., Weigert, M.G.: Miscoding caused by 5-fluorouracil. J. molec. Biol. 44, 363–375 (1969).
Sharp, D.G., Hook, A.E., Taylor, A.R., Beard, D., Beard, J.W.: Sedimentation characters and pH stability of the T2 bacteriophage of Escherichia coli. J. biol. Chem. 165, 259–270 (1946).
Wempen, I., Duschinsky, R., Kaplan, L., Fox, J.J.: Thiation of nucleosides. I. The synthesis of 5-fluoro-2-deoxycytidine and related compounds. J. Amer. chem. Soc. 83, 4755–4766 (1961).
Communicated by D. Goldfarb
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Andreev, V.M., Gonikberg, E.M. Influence of pH on phenotypic reversal of T4 phage Amber mutations by 5-fluorouracil. Molec. Gen. Genet. 112, 221–224 (1971). https://doi.org/10.1007/BF00269174
- Nutritive Medium
- Phenotypic Reversal
- Amber Mutation