A non-destructive, simple and accurate method of determining the relative growth rate (RGR) of the packed cell volume (PCV) of plant suspension cells in one Erlenmeyer flask at any time during the incubation period is described. The Erlenmeyer flask was tilted and the length of the chord formed by the surface of the packed cells across the bottom of the flask was measured. The chord length and the log PCV were correlated in a calibration line. The method enables the RGR during the exponential growth phase to be calculated by multiplying the slope of the linear part of the curve of the chord length in time with the slope of the calibration line. In order to investigate other growth parameters and to analyse the accuracy of the method statistically, a four-parameter function for the chord length and a computer program were used.
The RGR during the exponential growth phase of cell suspensions of Solanum tuberosum and Haplopappus gracilis appeared to be independent of the PCV of the inoculum. The method appeared to be sufficiently accurate.
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Alvey NG, Banfield CF, Baxter RI, Gower JC, Krzanowski WJ, Lane PW, Leech PK, Nelder JA, Payne RW, Phelps KM, Rogers CE, Ross GJS, Simpson HR, Todd AD, Wedderburn RWM, Wilkinson GN (1980) Genstat, a general statistical program. Numerical Algorithm Group Limited, Oxford
Gamborg OH, Miller RA, Ojima K (1963) Exp Cell Res 50: 151–155
Keen A, Gilissen LJW, Hänisch ten Cate ChH (1983) Technical and Preliminary Research Report No 97, Association Euratom-ITAL, Wageningen
King PJ, Street HE (1977) In: Street HE (ed) Botanical Monographs Vol. 11, Blackwell Scientific Publications, Oxford, pp 307–387
Kubek DJ, Shuler ML (1978a) Can J Bot 56: 2340–2343
Kubek DJ, Shuler ML (1978b) J Exp Bot 29: 511–523
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nelder JA (1961) Biometrics 17: 89–110
Nelder JA (1962) Biometrics 18: 614–616
Ranch JP, Giles KL (1980) Ann Bot 46: 667–683
Richards FJ (1959) J Exp Bot 10: 290–300
Street HE (1977) In: Street HE (ed) Botanical Monographs Vol 11, Blackwell Scientific Publications, Oxford, pp 61–102
Sung ZR (1976) Plant Physiol 57: 460–462
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Gilissen, L.J.W., Hänisch ten Cate, C.H. & Keen, B. A rapid method of determining growth characteristics of plant cell populations in batch suspension culture. Plant Cell Reports 2, 232–235 (1983). https://doi.org/10.1007/BF00269148
- Plant Cell
- Incubation Period
- Suspension Culture
- Erlenmeyer Flask
- Growth Parameter