Plant cells of tobacco (Nicotiana tabacum L.) were grown for several generations in suspension cultures. Cells were immobilized in continuous bioreactors in calcium alginate (Ca Alg) beads or in poly-L-lysine (PLL) encapsulated calcium alginatehydrogels. In each case, the cells were fed continuously a modified Linsmaier-Skoog plant cell culture medium. The bioreactor effluent was analyzed for total phenolic compounds. The net specific productivity of phenolics was calculated on a daily basis for several test runs. For comparison, productivity in suspension cultures was monitored. Productivity of suspended cells declined to zero within 9 d; both immobilized and encapsulated cells remained productive for 16 d following inoculation. Specific productivity by encapsulated cells was higher than that by immobilized cells; in both types similar rates of decline in productivity occurred.
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Communicated by G. C. Phillips
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Haigh, J.R., Linden, J.C. Phenolics production by encapsulated Nicotiana tabacum cells. Plant Cell Reports 8, 475–478 (1989). https://doi.org/10.1007/BF00269052
- Plant Cell
- Suspended Cell
- Phenolic Compound
- Suspension Culture