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Escherichia coli mutants uvr D and uvr E deficient in gene conversion of λ-heteroduplexes

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Summary

Calcium-treated cells of E. coli K-12 C600 were transfected with λ-heteroduplex DNA carrying the marker cIts857 in one strand and wildtype in the other. In single burst analyses of the phage progeny, 72–79% of the bursts were “pure” bursts containing either exclusively wildtype phage or exclusively mutant phage, indicating that conversion of the cIts857/+ mismatch to a homoduplex structure prior to replication occurred with this frequency. The r-strand1 appears to be “preferred”, since pure bursts of progeny with the r-strand genotype were almost twice as frequent as those with the l-strand genotype. Examination of the conversion frequency of a number of rec and uvr E. coli mutants showed that the mutants uvr D and uvr E are deficient in mismatch repair. conversion is reduced in the former by a factor of 2 and in the latter by a factor of 3.

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Nevers, P., Spatz, H. Escherichia coli mutants uvr D and uvr E deficient in gene conversion of λ-heteroduplexes. Molec. Gen. Genet. 139, 233–243 (1975). https://doi.org/10.1007/BF00268974

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Keywords

  • Gene Conversion
  • Mismatch Repair
  • Conversion Frequency
  • Single Burst
  • Phage Progeny