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Temperature-dependent conversion of sexual agglutinability in Saccharomyces cerevisiae

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Summary

Temperature dependency of sexual agglutinability in Saccharomyces cerevisiae was found. In almost all strains tested that were derived from several different sources, the agglutinability was constitutive when grown at 25° C but inducible when grown at 36° C, suggesting that the temperature-dependent conversion of sexual agglutinability is general nature in Saccharomyces. Cycloheximide and 8-hydroxyquinoline inhibited completely both cell division and the conversion of the agglutinability from constitutive to inducible type. N-Hydroxyurea and 5-fluorouracil which allowed cell growth to some extent inhibited the conversion slightly. Hence, the conversion of the agglutinability from constitutive to inducible type may be achieved in cells newly born after temperature shift. The reverse conversion of the agglutinability was gradual in comparison with the conversion from constitutive to inducible type. This conversion of the agglutinability was regulated by a single gene closely linked to mating type locus, which is recognizable by using a temperature-independent constitutive strain.

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Communicated by F. Kaudewitz

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Doi, S., Yoshimura, M. Temperature-dependent conversion of sexual agglutinability in Saccharomyces cerevisiae . Molec. Gen. Genet. 162, 251–257 (1978). https://doi.org/10.1007/BF00268850

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Keywords

  • Cell Growth
  • Cell Division
  • Saccharomyces Cerevisiae
  • Saccharomyces
  • Single Gene