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A mutation in Escherichia coli enhancing the UV-mutability of phage λ but not of its infectious DNA in a spheroplast assay

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Summary

A mutant (called mul) has been isolated from E. coli AB1157 which increases up to 13 fold the rate of clear plaque mutations of extracellularly UV-irradiated phage λ. The mul-mutation does not affect the UV-mutability of T4rII mutants or various bacterial markers and therefore seems to act specifically on UV-irradiated phage λ. However, when spheroplasts prepared from mul cells were infected with either irradiated or unirradiated λ-DNA equal frequencies of clear plaques were produced. As there is indirect evidence (host cell reactivation) that the spheroplasts do not significantly exclude irradiated phage DNA it seems that the mul phenotype can be expressed only in complete cells.

The mutation responsible for the mul phenotype has been mapped by conjugation and transduction to be located between the markers pyrE and ilv and probably marks a new gene. The mul mutation does not increase the UV-sensitivity of the cells nor does it affect their ability to perform host cell reactivation. The presence of a recA allel in a mul mutant abolishes the UV-mutability of phage λ.

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References

  1. Adams, M. H.: Bacteriophages. New York-London: Interscience Publ. 1959.

  2. Clark, A. J., Margulies, A. D.: Isolation and characterisation of recombination deficient mutants of E. coli K12. Proc. nat. Acad. Sci. (Wash.) 53, 451–459 (1965).

  3. Defais, N., Fauquet, P., Radman, M., Errera, M.: Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systems. Virology 43, 495–503 (1971).

  4. Dove, W. F.: The genetics of the lambdoid phages. Ann. Rev. Genet. 2, 305–340 (1968).

  5. Drake, J. W.: Properties of ultraviolet-induced rII mutants of bacteriophage T4. J. molec. Biol. 6, 268–283 (1963).

  6. —: Ultraviolet mutagenesis in bacteriophage T4. I. Irradiation of extracellular phage particles. J. Bact. 91, 1775–1780 (1966).

  7. Echols, H., Gingery, R.: Mutants of bacteriophage defective in vegetative genetic recombination. J. molec. Biol. 34, 239–249 (1968).

  8. Ellmauer, H., Kaplan, R. W.: Auslösung von Klarplaquemutationen durch UV-Bestrahlung des freien Phagen Kappa von Serratia marcescens. Naturwissenschaften 46, 150 (1959).

  9. Harm, W., Rupert, C. S.: Infection of transformable cells of Hemophilus influence by bacteriophage and bacteriophage DNA. Z. Vererbungsl. 94, 336–348 (1963).

  10. Howard-Flanders, P., Boyce, R. P.: DNA repair and genetic recombination studies on mutants of Escherichia coli defective in these processes. Radiat. Res., Suppl. 6, 156–184 (1966).

  11. Kaiser, A. D.: Mutations in a temperate bacteriophage affecting its ability to lysogenise Escherichia coli. Virology 3, 42–61 (1957).

  12. Kaplan, R. W., Lohr, R. v., Brendel, M.: Influence of the host on the induction and expression of mutations in phage Kappa. In: Mutation as a cellular process, a Ciba foundation symposium; ed. by G. E. W. Wolstenholme and M. O'Connor, p. 3–13, London (1969).

  13. Kriviski, A. A., Tichomirowa, L. P.: Der Einfluß chemischer Agentien auf die letalen und mutagenen Effekte der UV-Bestrahlung des extrazellulären Phagen Cd. Studia Biophysica 19, 103–121 (1970).

  14. Miura, A., Tomizawa, J.: Studies on radiation-sensitive mutants of Escherichia coli. III. Participation of the rec-system in induction of mutation by ultraviolet irradiation. Molec. gen. Genet. 103, 1–10 (1968).

  15. —: Mutation and recombination of bacteriophage λ: Effect of ultraviolet radiation. Proc. nat. Acad. Sci. (Wash.) 67, 1722–1726 (1970).

  16. Putte, P. van de, Sluis, C. A. van, Dillewijn, J. van, Rörsch, A.: The location of genes controlling radiation sensitivity in Escherichia coli. Mutation Res. 2, 97–110 (1965).

  17. Radman, M., Cordone, L., Krsmanovic-Simic, D., Errera, M.: Complementary action of recombination and excision in the repair of ultraviolet irradiation damage to DNA. J. molec. Biol. 49, 203–212 (1970).

  18. Setlow, J. K.: The molecular basis of biological effects of UV-irradiation and photoreactivation. In: Current topics in radiation research, ed. by M. Ebert and A. Howard, vol. II, p. 195–248. Amsterdam: North Holland Publ. 1966.

  19. Shulman, M. J., Hallick, L. M., Echols, H., Signer, E. R.: Properties of recombination deficient mutants of bacteriophage Lambda. J. molec. Biol. 52, 501–520 (1970).

  20. Sussman, R., Jacob, F.: Sur un système de répression thermosonsible chez le bactériophage λ d'Escherichia coli. C. R. Acad. Sci. (Paris) 254, 1517–1519 (1962).

  21. Taylor, A. L., Trotter, C. D.: Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353 (1967).

  22. Wackernagel, W.: Erhöhte UV-Mutabilität des Phagen Lambda in Mutanten von E. coli. Zbl. Bakt. I Orig. 212, 389 (1969).

  23. Wackernagel, W.: An improved spheroplast assay for λ-DNA and the influence of the bacterial genotype on the transfection rate. Virology, in press.

  24. Weigle, J. J.: Induction of mutation in a bacterial virus. Proc. nat. Acad. Sci. (Wash.) 39, 628–636 (1953).

  25. Weil, J., Signer, E. R.: Recombination in bacteriophage λ: II. Site specific recombination promoted by the integration system. J. molec. Biol. 34, 273–279 (1968).

  26. Winkler, U.: Wirtsreaktivierung von extrazellulär strahleninduzierten Prämutationen im Serratia-Phagen Kappa. 2. Über Prämutationen vollständig löschende Bakterienmutanten. Z. Vererbungsl. 97, 29–39 (1965).

  27. —: Mutants of Serratia marcescens defective or superactive in the release of a nuclease. In: Molecular genetics, ed. by H. G. Wittmann and H. Schuster, p. 187–201. Berlin-Heidelberg-New York: Springer 1968.

  28. Witkin, E. M., Theil, E. C.: The effect of post treatment with chloramphenicol on various UV-induced mutations in E. coli. Proc. nat. Acad. Sci. (Wash.) 50, 226–231 (1960).

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Part of this work is from the thesis of the first author in partial fullfilment of the requirements for the doctoral degree of the University of Bochum. Some of the results have already been presented at the “32. Kongreß für Hygiene und Mikrobiologie” at Münster, 1969.

Communicated by G. Bertani

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Wackernagel, W., Winkler, U. A mutation in Escherichia coli enhancing the UV-mutability of phage λ but not of its infectious DNA in a spheroplast assay. Molec. Gen. Genet. 114, 68–79 (1972). https://doi.org/10.1007/BF00268748

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Keywords

  • Host Cell
  • Indirect Evidence
  • Cell Reactivation
  • Equal Frequency
  • Complete Cell