Twenty-seven spontaneous mutants were selected for streptomycin (50 μg/ml) resistance from Bacillus stearothermophilus strain 799. All of them were derived from independent mutational origins. Of these, ten mutants (about 35%) were found to be resistant to streptomycin, thirteen (about 50%) were fully and four (about 15%) were partially dependent on streptomycin for growth. The last mutants grow poorly without streptomycin but grow well with it. One of the ten resistant mutants isolated was partially sensitive to higher concentrations of streptomycin, while all the rest showed good growth with up to 500 μg/ml streptomycin. From some of the dependent mutants, spontaneous revertants which have lost the property of streptomycin dependence were isolated. 30 S ribosomal proteins from these five classes of mutants were analyzed by two-dimensional gel electrophoresis. Thus S12 was found to be affected in some of the resistant and fully dependent mutants, while S4 or S5 were altered in some of the independent revertants. One of the mutants partially dependent on streptomycin were found to have an alteration in S5. Another mutant partially sensitive to higher concentrations of streptomycin appears to have an alteration also in S5. The last two mutants are new classes of streptomycin mutants.
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Birge, E. A., Kurland, C. G.: Altered ribosomal protein in streptomycin-dependent Escherichia coli. Science 166, 1282–1286 (1969)
Birge, E. A., Kurland, C. G.: Reversion of a streptomycin dependent strain of Escherichia coli. Molec. gen. Genet. 109, 356–369 (1970)
Deusser, E., Stöffler, G., Wittmann, H. G., Apirion, D.: Ribosomal proteins, XII. Altered S4 proteins in Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 109, 298–302 (1970)
Funatsu, G., Puls, W., Schiltz, E., Reinbolt, J., Wittmann, H. G.: Ribosomal proteins, XXXI. Comparative studies on S4 proteins of six Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 115 131–139 (1972)
Funatsu, G., Wittmann, H. G.: Ribosomal proteins, XXXIII. Location of amino acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin J. molec. Biol. 68, 547–550 (1972)
Hardy, S. J. S., Kurland, C. G., Voynow, P., Mora, G.: The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry 8, 2897–2905 (1969)
Hasenbank, R., Guthrie, C., Stöffler, G., Wittmann, H. G., Rosen, L., Apirion, D.: Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Molec. gen. Genet. 127, 1–18 (1973)
Higo, K., Held, W., Kahan, L., Nomura, M.: Functional correspondence between 30S ribosomal proteins of Escherichia coli and Bacillus stearothermophilus. Proc. nat. Acad. Sci. (Wash.) 70, 944–948 (1973)
Hindennach, I., Stöffler, G., Wittmann, H. G.: Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli. Eur. J. Biochem. 23, 7–11 (1971)
Isono, K., Isono, S., Stöffler, G., Visentin, L. P., Yaguchi, M., Matheson, A. T.: Correlation between 30S ribosomal proteins of Bacillus stearothermophilus and Escherichia coli. Molec. gen. Genet. 127, 191–195 (1973)
Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins, VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401–412 (1970)
Kreider, G., Brownstein, B. L.: A mutation suppressing streptomycin dependence, II. An altered protein in the 30S ribosomal subunit. J. molec. Biol. 61, 135–142 (1971)
Kreider, G., Brownstein, B. L.: Ribosomal proteins involved in the suppression of streptomycin dependence in Escherichia coli. J. Bact. 109, 780–785 (1972)
Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli. Nature (Lond.) 222, 333–339 (1969)
Stöffler, G., Deusser, E., Wittmann, H. G., Apirion, D.: Ribosomal proteins, XIX. Altered S5 ribosomal proteins in an Escherichia coli revertant from streptomycin dependence to independence. Molec. gen. Genet. 111, 334–341 (1971)
Wittmann, H. G., Stöffler, G., Hindennach, I., Kurland, C. G., Randall-Hazelbauer, L., Birge, E. A., Nomura, M., Kaltschmidt, E., Mizushima, S., Traut, R. R., Bickle, T. A.: Correlation of 30 S ribosomal proteins of Escherichia coli isolated in different laboratories. Molec. gen. Genet. 111, 327–333 (1971)
Paper No. 95 on “Ribosomal Proteins”; Preceding paper is by Richter and Isono, FEBS Letters, in press.
Communicated by E. Bautz
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Isono, K. Altered 3OS ribosomal proteins in streptomycin resistant, dependent and independent mutants of Bacillus stearothermophilus . Molec. Gen. Genet. 133, 77–86 (1974). https://doi.org/10.1007/BF00268679
- Ribosomal Protein
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