Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Use of RP4-prime plasmids constructed in vitro to promote a polarized transfer of the chromosome in Escherichia coli and Rhizobium meliloti

Summary

RP4-prime plasmids containing chromosomal fragments of either Escherichia coli or Rhizobium meliloti were constructed in vitro. When introduced into E. coli or R. meliloti respectively, they promoted a polarized transfer of the chromosome as demonstrated either by the gradient of transfer of various markers or by the study of the genetic constitution of recombinants. In E. coli, mobilization was shown to be dependent upon the presence of a functional rec A system. Inheritance of markers was due to their integration into the chromosome of the recipient as shown by the need for a functional rec A system in the recipient E. coli or by mobilization of recessive markers in R. meliloti. The system described could be applied to genetic mapping in any Gram negative bacteria.

This is a preview of subscription content, log in to check access.

References

  1. Austin, S., Scaife, J.: A new method for selecting RNA polymerase mutants. J. Mol. Biol. 49, 263–267 (1973)

  2. Bachman, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K12. Bacteriol. Rev. 40, 116–167 (1976).

  3. Barth, P.T., Grinter, N.J.: Map of plasmid RP4 derived by insertion of transposon C. J. Mol. Biol. 113, 455–474 (1977)

  4. Beringer, J.E., Hoggan, S.A., Johnston, A.W.B.: Linkage mapping in Rhizobium leguminosarum by means of R plasmid mediated recombination. J. Gen. Microbiol. 104, 201–207 (1978)

  5. Boucher, C., Bergeron, B., Barate de Bertalmio, M., Dénarié, J.: Introduction of bacteriophage Mu into Pseudomonas solanacearum and Rhizobium meliloti using the R factor RP4. J. Gen. Microbiol. 98, 253–263 (1977)

  6. Casse, F., Boucher, C., Julliot, J.S., Michel, M., Dénarié, J.: Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. J. Gen. Microbiol. (in the press)

  7. Clewell, B.D., Helinski, D.R.: Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc. Natl. Acad. Sci. U.S.A. 62, 1159–1166 (1969)

  8. Cohen, S.N., Chaang, A.C.Y., Hsu, L.: Non chromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R factor DNA. Proc. Natl. Acad. Sci. U.S.A. 69, 2110–2114 (1972)

  9. Currier, T.C., Nester, E.W.: Isolation of covalently closed circular DNA of high molecular weight from bacteria. Anal. Biochem. 76, 431–441 (1976)

  10. Datta, N., Hedges, R.W.: Host ranges of R factors. J. Gen. Microbiol. 70, 453–460 (1972)

  11. Dénarié, J., Rosenberg, C., Bergeron, B., Boucher, C., Michel, M., Barate de Bertalmio, M.: Potential of RP4:: Mu plasmids for in vivo genetic engineering in Gram-negative bacteria. In: DNA insertion elements, plasmids and episomes (A.I. Bukhari, J.A. Shapiro and S.L. Adhya, eds.). New York: Cold Spring Harbor Laboratory 1977

  12. Haas, D., Holloway, B.W.: R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol. Gen. Genet. 144, 243–251 (1976)

  13. Hayes, W.: The genetics of bacteria and their viruses, 2nd edit., pp. 679–696. Oxford-Edinburgh: Blackwell Scientific Publications 1968

  14. Heffron, F., Bedinger, P., Champoux, J.J., Falkow, S.: Deletions affecting the transposition of an antibiotic resistance gene. Proc. Natl. Acad. Sci. U.S.A. 74, 702–706 (1977)

  15. Humphreys, G.O., Willshaw, G.A., Anderson, E.S.: A simple method for the preparation of large quantities of pure plasmid DNA Biochim. Biophys. Acta 383, 457–463 (1975)

  16. Jacob, A.E., Cresswell, J.M., Hedges, R.W., Coetzee, J.N., Beringer, J.E.: Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4. Mol. Gen. Genet. 147, 315–323 (1976)

  17. Kleckner, N., Roth, J., Botstein, D.: Genetic engineering in vivo using translocatable drug resistance elements. New Methods in bacterial genetics. J. Mol. Biol. 116, 125–159 (1977)

  18. Klotz, L.C., Zimm, B.H.: Size of DNA determined by viscoclastic measurements: results on bacteriophages, Bacillus subtilis and Escherichia coli. J. Mol. Biol. 72, 779–800 (1972)

  19. Kondorosi, A., Kiss, G.B., Forrai, T., Vincze, E., Banfalvi, Z.: Circular linkage map of the Rhizobium meliloti chromosome. Nature 268, 525–527 (1977)

  20. Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206 (1955)

  21. Low, K.B.: Escherichia coli K12 F-prime factors, old and new. Bacteriol. Rev. 36, 587–607 (1972)

  22. Meade, H.M., Signer, E.R.: Genetic mapping of Rhizobium meliloti. Proc. Natl. Acad. Sci. U.S.A. 74, 2076–2078 (1977)

  23. Monod, J., Cohen-Bazire, G., Cohn, M.: Sur la biosyntèse de la β galactosiclase (lactase) chez Escherichia coli. La spécificité de l'induction. Biochim. Biophys. Acta 7, 585–589 (1951)

  24. Prakash, R.K., Hooykaas, P.J.J., Ledeboer, A.M., Kijne, J., Schilperoort, R.A., Nuti, M.P., Lepidi, A.A., Casse, F., Boucher, C., Julliot, J.S., Denarie, J.: Detection, isolation and characterization of large plasmids in Rhizobium. In: Proc. of the 3rd Internat. Symposium on Nitrogen Fixation (N. Newton and Orme Johnson), eds. Madison: University Park Press (in the press)

  25. Scherrer, A., Denarie, J.: Symbiotic properties of some auxotrophic mutants of Rhizobium meliloti and of their prototrophic revertants. In: Biological nitrogen fixation in natural and agricultural habitats (T.A. Lie and E.G. Mulder, eds.), pp. 39–49. The Hague: Martinus Nijhoff 1971

  26. Stanisich, V.A., Holloway, B.W.: Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genet. Res. 17, 169–172 (1971)

  27. Tanaka, T., Weisblum, B.: Construction of colicin E1-R factor composite plasmid in vitro: Means for amplification of deoxyribonucleic acid. J. Bacteriol. 121, 354–362 (1975)

  28. Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with Eco R1 restriction endonuclease. J. Mol. Biol. 91, 315–328 (1975)

  29. Towner, K.J., Vivian, A.: RP4-mediated conjugation in Acinetobacter calcoaceticus. J. Gen. Microbiol. 93, 355–366 (1976)

  30. Van Vliet, F., Silva, B., Van Montagu, M., Schell, J.: Transfer of RP4:: Mu plasmids to Agrobacterium tumefaciens. Plasmid 1, 446–455 (1978)

  31. Watson, J.M., Holloway, B.W.: Chromosome mapping in Pseudomonas aeruginosa PAT. J. Bacteriol. 133, 1113–1125 (1978)

  32. Wijffelman, C., Gassler, M., Stevens, W.F., Van de putte, P.: On the control of transcription of bacteriophage Mu. Mol. Gen. Genet. 131, 85–96 (1974)

Download references

Author information

Correspondence to Pierre Boistard.

Additional information

Communicated by J. Schell

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Julliot, J.S., Boistard, P. Use of RP4-prime plasmids constructed in vitro to promote a polarized transfer of the chromosome in Escherichia coli and Rhizobium meliloti . Molec. Gen. Genet. 173, 289–298 (1979). https://doi.org/10.1007/BF00268639

Download citation

Keywords

  • Genetic Mapping
  • Rhizobium
  • Negative Bacterium
  • Genetic Constitution
  • Chromosomal Fragment