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Molecular and General Genetics MGG

, Volume 135, Issue 3, pp 185–190 | Cite as

Competition for DNA polymerase III between the chromosome and the R-factor R1

  • U. Marie Nordström
  • Birgitta Enberg
  • Kurt Nordström
Article

Summary

The R-factor R1 is normally present in a low number of copies per genome in Escherichia coli K12. This number may be incrased several-fold either by lowering the growth rate or by mutation (copy mutants); these two effects act synergistically. The effect of the copy number on growth rate was tested in a strain with a temperaturesensitive DNA-polymerase III (dnaE(ts)) using wild type and copy mutants of the R-factor R1. The presence of the copy mutant R1drd-19B2 increased the temperature-sensitivity by 1.5–2°C and reduced the growth rate at temperatures close to the maximum temperature. This growth rate reduction was greater the higher the number of R1 copies/genome. The results indicate that DNA-polymerase III is involved in the replication of the plasmid R1 and that R1 and the chromosome compete for the enzyme.

Keywords

Enzyme Growth Rate Escherichia Coli Rate Reduction Growth Rate Reduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • U. Marie Nordström
    • 1
  • Birgitta Enberg
    • 1
  • Kurt Nordström
    • 1
  1. 1.Department of MicrobiologyUniversity of UmeåUmeåSweden

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