The uptake of L-[methylene 14C]-tryptophan from culture medium and the subsequent incorporation of the radiolabel into canthin-6-one, 1-hydroxycanthin-6-one and 1-methoxycanthin-6-one has been demonstrated in cell suspension cultures of Ailanthus altissima. Efficient incorporation has been shown to depend significantly on the time of feeding. Furthermore, feeding of L-tryptophan, at levels of 500 mg/l resulted in improved alkaloid yields, particularly when fed during the lag phase of the growth cycle.
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Anderson, L.A., Hay, C.A., Roberts, M.F. et al. Studies on Ailanthus altissima cell suspension cultures. Plant Cell Reports 5, 387–390 (1986). https://doi.org/10.1007/BF00268609
- Cell Suspension
- Suspension Culture