Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Studies on Ailanthus altissima cell suspension cultures

Precursor feeding of L-[methylene-14C]tryptophan and L-tryptophan

  • 39 Accesses

  • 16 Citations

Abstract

The uptake of L-[methylene 14C]-tryptophan from culture medium and the subsequent incorporation of the radiolabel into canthin-6-one, 1-hydroxycanthin-6-one and 1-methoxycanthin-6-one has been demonstrated in cell suspension cultures of Ailanthus altissima. Efficient incorporation has been shown to depend significantly on the time of feeding. Furthermore, feeding of L-tryptophan, at levels of 500 mg/l resulted in improved alkaloid yields, particularly when fed during the lag phase of the growth cycle.

This is a preview of subscription content, log in to check access.

References

  1. Anderson LA, Harris A and Phillipson JD (1983) J Nat Prod 46: 374–378

  2. Anderson LA, Phillipson JD and Roberts MF (1985a) “Biosynthesis of Secondary Products by Cell Cultures of Higher Plants” In: Fiechter A (ed) Advances in Biochemical Engineering/Biotechnology, Volume 31, Springer-Verlag, Berlin, Heidelberg, New York pp 1–36

  3. Anderson LA, Phillipson JD and Roberts MF (1985b) J Pharm Pharmacol 37: 45P

  4. Anderson LA, Phillipson JD and Roberts MF (1986a) “Aspects of Alkaloid Production by Plant Cell Cultures” In: Morris P, Scragg A, Stafford A and Fowler M (eds) Secondary Metabolism in Plant Cell Cultures, Cambridge University Press, Cambridge

  5. Anderson LA, Phillipson JD and Roberts MF (1986b) “Alkaloid Production by Plant Cells” In: Proceedings of ‘Process Possibilities for Plant And Animal Cell Cultures’ UMIST, Manchester

  6. Graffeo AP and Karger BL (1976) Clin Chem 22(2): 184–187

  7. Hay CA, Anderson LA, Roberts MF and Phillipson JD (1986) Plant Cell Reports 5(1): 1–4

  8. Hegnauer R (1973) Chemotaxonomie der Pflanzen Vol. 6, Birkhauser Verlag, Basel and Stuttgart p387

  9. Ohmoto T., Koike K and Sakamoto Y (1981) Chem Pharm Bull 29: 390–395

  10. Ohmoto T and Koike K (1984) Chem Pharm Bull 32: 170–173

  11. Varga E, Szendrei K, Reisch J and Maroti G (1980) Planta Medica 40: 337–339

  12. Varga E, Szendrei K, Reisch J and Maroti G (1981) Fitoterapia 4: 183–186

  13. Veliky IA (1972) Phytochemistry 11: 1405–1406

  14. Veliky IA and Barber KM (1975) Lloydia 38: 125–130

  15. Wijnsma R, van der Leer T, Verpoorte R, Harkes PAA, de Jong PJ and Baerheim Svendsen A (1986) Plant Cell Reports, in press

  16. Zenk MH, El-Shagi H, Arens H, Stockigt J, Weiler EW and Deus B (1977) In: Barz W, Reinhard E and Zenk MH (eds) Plant Tissue Culture and its Bio-technological Application, Springer-Verlag, Berlin, Heidelberg and New York pp27–43

Download references

Author information

Correspondence to L. A. Anderson.

Additional information

Communicated by M. H. Zenk

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Anderson, L.A., Hay, C.A., Roberts, M.F. et al. Studies on Ailanthus altissima cell suspension cultures. Plant Cell Reports 5, 387–390 (1986). https://doi.org/10.1007/BF00268609

Download citation

Keywords

  • Methylene
  • Cell Suspension
  • Tryptophan
  • Alkaloid
  • Suspension Culture