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Some characteristics of peroxidase secreted by cotton ovule cultures

Abstract

Cotton (Gossypium hirsutum L.) ovule cultures secreted a soluble peroxidase into the surrounding medium, resulting in a 200-fold increase in this activity during the 30-day growth period. The peroxidase activity was thermostable from 4°C to 60°C and displayed a pH optimum of 5.5 to 6.0. The ovule peroxidase was susceptible to periodate treatment and very resistant to protease digestion. The data suggest that the peroxidase activity is a glycoprotein. Interpretation of peroxidase data may be complicated by the presence of phenol oxidase activity in the same preparations. The presence of phenol oxidases was ruled out by the inaction of a tyrosinase-specific inhibitor, tropolone.

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Abbreviations

PMSF:

phenylmethylsulfonyl fluoride

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Communicated by I.K. Vasil

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Mellon, J.E. Some characteristics of peroxidase secreted by cotton ovule cultures. Plant Cell Reports 5, 338–341 (1986). https://doi.org/10.1007/BF00268596

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Keywords

  • Phenol
  • Periodate Treatment
  • Peroxidase Activity
  • Growth Period
  • Oxidase Activity