Cotton (Gossypium hirsutum L.) ovule cultures secreted a soluble peroxidase into the surrounding medium, resulting in a 200-fold increase in this activity during the 30-day growth period. The peroxidase activity was thermostable from 4°C to 60°C and displayed a pH optimum of 5.5 to 6.0. The ovule peroxidase was susceptible to periodate treatment and very resistant to protease digestion. The data suggest that the peroxidase activity is a glycoprotein. Interpretation of peroxidase data may be complicated by the presence of phenol oxidase activity in the same preparations. The presence of phenol oxidases was ruled out by the inaction of a tyrosinase-specific inhibitor, tropolone.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bredemeijer GMM, Burg HCJ, Sree Ramulu K, Dijkhuis P (1985) Acta Bot. Neerl. 34:325–335
Chibbar RN, Cella R, Albani D, van Huystee RB (1984) J. Exp. Bot. 35:1846–1852
Egley GH, Paul Jr. RN, Duke SO, Vaughn KC (1985) Plant Cell Environ. 8:253–260
Egley GH, Paul Jr. RN, Vaughn KC, Duke SO (1983) Planta 157:224–232
Fry SC (1980) Phytochemistry 19:735–740
Grison R, Pilet PE (1985) Phytochemistry 24:2519–2521
Heidrich E, Lorenz G, Schreier P (1983) Food Chem. 10:285–296
Kahn V (1985) Phytochemistry 24:915–920
Lamport DTA (1980) In: Stumpf PK, Conn EE (eds) The biochemistry of plants, vol III, Preiss J (ed), Carbohydrates: structure and function, chpt 13, Academic, New York London Toronto Sydney San Francisco, pp 501–541
Mellon JE (1985) Plant Physiol. 77:S-69
Mellon JE, Lee LS (1985) Physiol. Plant Pathol. 27:281–288.
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Anal. Biochem. 150:76–85
Stewart JMcD, Hsu CL (1977) Planta 137:113–117
van Huystee RB, Turcon G (1973) Can. J. Bot. 51:1169–1175
Wise B, Morrison M (1971) Phytochemistry 10:2355–2359
Communicated by I.K. Vasil
About this article
Cite this article
Mellon, J.E. Some characteristics of peroxidase secreted by cotton ovule cultures. Plant Cell Reports 5, 338–341 (1986). https://doi.org/10.1007/BF00268596
- Periodate Treatment
- Peroxidase Activity
- Growth Period
- Oxidase Activity