Using various replication mutants of E. coli, the host genes that participate in the replication of some K12-specific single-stranded DNA phages have been determined. Functional products of dnaE,-F,-G and -Z genes are required for the multiplication of ϕK, whereas dnaA,-B,-C(D), H,-I and -P are dispensable for viral replication. In contrast with polB, recA, B, C, or xth functions, host rep activity is essential for ϕK. At the restrictive temperature, the yield of ϕK was markedly reduced in the ligts7 mutant and partially decreased in a polA ts strain. The phage ϕK is thus less dependent on the host cells than ϕX174 and ϕA which require additionally the dnaB,-C(D) and -H functions. Replication of phage St-1 depends on dnaG and -Z gene products, but not on dnaP function. Although not much affected in polA ts host, growth of St-1 was significantly diminished in dnaF or ligts7 mutants.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bouché, J.-P., Zechel, K., Kornberg, A.: dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coli phage DNA to its duplex replicative form. J. biol. Chem. 250, 5995–6001 (1975)
Bowes, J.M.: Replication of bacteriophage St-1 in Escherichia coli strains temperature sensitive in DNA synthesis. J. Virol. 13, 1400–1403 (1974)
Bowes, J.M., Dowell, C.E.: Purification and some properties of bacteriophage St-1. J. Virol. 13, 53–61 (1974)
Bradley, D.E.: A comparative study of some properties of the ϕX174 type bacteriophages. Canad. J. Microbiol. 16, 965–971 (1970)
Denhardt, D.T., Iwaya, M., Larison, L.L.: The rep mutation. II. Its effect on Escherichia coli and on the replication of bacteriophage ϕX174. Virology 49, 486–496 (1972)
Godson, G.N.: Evolution of ϕX174. Isolation of four new ϕX-like phages and comparison with ϕX174. Virology 58, 272–289 (1974)
Schekman, R., Weiner, A., Kornberg, A.: Multienzyme systems of DNA replication. Science 186, 987–993 (1974)
Schekman, R., Wickner, W., Westergaard, O., Brutlag, D., Geider, K., Bertsch, L.L., Kornberg, A.: Initiation of DNA synthesis: Synthesis of ϕX174 replicative form requires RNA synthesis resistant to rifampicin. Proc. nat. Acad. Sci. (Wash.). 69, 2691–2695 (1972)
Taketo, A.: Sensitivity of Escherichia coli to viral nucleic acid. VI. Capacity of dna mutants and DNA polymerase-less mutants for multiplication of ϕX174. Molec. gen. Genet. 122, 15–22 (1973)
Taketo, A.: Properties of bacterial virus ϕA and its DNA. J. Biochem. (Tokyo) 75, 951–960 (1974)
Taketo, A.: Replication of ϕA and ϕX174 in Escherichia coli mutants thermosensitive in DNA synthesis. Molec. gen. Genet. 139, 285–291 (1975a)
Taketo, A.: Inducibility of lambda phage development in Escherichia coli mutants thermosensitive for DNA replication. Z. Naturforsch. 30c, 800–803 (1975b)
Taketo, A.: Ba2+-induced competence for trasfecting DNA. Z. Naturforsch. 30c, 520–522 (1975c)
Taketo, A., Yasuda, S., Sekiguchi, M.: Initial step of excision repair in Escherichia coli: Replacement of defective function of uvr mutants by T4 endonuclease V. J. molec. Biol. 70, 1–14 (1972)
Truitt, C.L., Walker, J.R.: Growth of phages λ, ϕX174, and M13 requires the dnaZ (previously dnaH) gene product of Escherichia coli. Biochem. biophys. Res. Commun. 61, 1036–1042 (1974)
Wickner, S., Hurwitz, J.: Association of ϕX174 DNA-dependent ATPase activity wth an Escherichia coli protein, replication factor Y, required for in vitro synthesis of ϕX174 DNA. Proc. nat. Acad. Sci. (Wash.) 72, 3342–3346 (1975)
Yajko, D.M., Weiss, B.: Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 72, 688–692 (1975)
Communicated by G. Bertani
About this article
Cite this article
Taketo, A. Host genes involved in the replication of single-stranded DNA phage ϕK. Molec. Gen. Genet. 148, 25–29 (1976). https://doi.org/10.1007/BF00268542
- Gene Product
- Host Cell
- Viral Replication
- Host Gene
- Functional Product