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Revertants of double Opal-mutants of bacteriophage T4

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Summary

Revertants of double opal-mutants of bacteriophage T4 have been obtained. The properties of these revertants suggest that reversion of double opal-mutants is effected by the activity of some genesuppressor appeared in the phage genome. Restriction of these revertants by streptomycin-resistant bacterial strains shows that the suppression of the opal-mutants is realized at translation.

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References

  1. Chan, T.S., Garen, A.: Amino acid substitutions resulting from suppression of nonsense mutants. V. Tryptophan insertion by the su 9t gene, a suppressor of the UGA nonsense triplet. J. molec. Biol. 49, 231–234 (1970)

  2. Comer, M.M., Guthrie, C., McClain, W.H.: An ochre suppressor of bacteriophage T4 that is associated with a transfer RNA. J. molec. Biol. 90, 665–676 (1974)

  3. Elsevierse, D., Gorini, L.: Directed selection of mutants restricting efficiency of suppression and misreading levels in E. coli B. Molec. gen. Genet. 137, 277 (1975)

  4. Gorini, L.: Informational suppression. Annu. Rev. Genet. 4, 107–134 (1970)

  5. Hirsh, D.: Tryptophan transfer RNA as the UGA suppressor. J. molec. Biol. 58, 439–458 (1971)

  6. Hirsh, D., Gold, L: Translation in vitro of the UGA triplet by tryptophan transfer RNA. J. molec. Biol. 58, 459–468 (1971)

  7. Maisurian, A.N., Pozdniakov, V.N.: Suppression of temperature sensitive mutants of bacteriophage T4 by bacterial opal suppressors. Molec. gen. Genet. 112, 91–92 (1971)

  8. Scherberg, N., Weiss, S.: Detection of bacteriophage T4 and T5 coded transfer RNA. Proc. nat. Acad. Sci. (Wash.) 67, 1164–1167 (1970)

  9. Scherberg, N., Weiss, S.: T4 transfer RNA's: Codon recognition and translational properties. Proc. nat. Acad. Sci. (Wash.) 69, 1114–1118 (1972)

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Communicated by D. Goldfarb

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Pozdnlakov, V.N., Maisurian, A.N. Revertants of double Opal-mutants of bacteriophage T4. Molec. Gen. Genet. 149, 357–358 (1976). https://doi.org/10.1007/BF00268538

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Keywords

  • Bacterial Strain
  • Phage Genome