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Somatic embryogenesis from hypocotyl callus cultures of Digitalis obscura L.

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Hypocotyl-derived calli obtained in agar solidified medium with several growth regulator combinations gave rise to proembryonal masses and globular embryos when transferred to liquid media with lower growth regulator and higher NH4HO3 levels. By transferring cultures from liquid media to different solidified media, new embryo formation took place, but further development of these embryos or those previously induced depended on the characteristics of these media. Normal development was only achieved on 8 g/l agar solidified medium without growth regulators. Typical cotyledonary embryos developed into whole plants when transferred to this same medium.

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casein hydrolysate


coconut milk


2,4-dichlorophenoxyacetic acid






naphthaleneacetic acid


indoleacetic acid


  1. Ammirato PV (1977) Plant Physiol 59: 579–586

  2. Ammirato PV, Steward FC (1971) Bot Gaz 132: 149–158

  3. Arrillaga I, Brisa MC, Segura J (1986) J Plant Physiol 124: 425–430

  4. Cronauer S, Krikorian AD (1983) Plant Cell Reports 2: 289–291

  5. Diettrich B, Steup C, Neumann D, Scheibner C, Reinbothe C, Luckner M (1986) J Plant Physiol 124: 441–453

  6. Garve R, Luckner M, Vogel E, Tewes A, Nover L (1980) Planta Med 40: 92–103

  7. Hussey G (1986) In: Yeoman MN (ed) Plant Cell Culture Technology, Blackwell Scientific Publications, Oxford, pp 29–66

  8. Kuberski C, Scheibner H, Steup C, Diettrich B, Luckner M (1984) Phytochem 23: 1407–1412

  9. Luckner M, Diettrich B (1985) In: Neumann KH, Barz W, Reinhard E (eds) Primary and Secondary Metabolism of Plant Cell Cultures, Springer-Verlag, Berlin Heidelberg New York Tokyo, pp 154–163

  10. Murashige T, Skoog F (1962) Physiol Plantarum 15: 473–497

  11. Pérez-Bermúdez P, Cornejo MJ, Segura J (1983) Plant Sci Lett 30: 77–82

  12. Street JE (1978) In: Schutte HR, Gross D (eds) Regulation of Developmental Processes in Plants, VEB Kongress-und Werbedruk, Oberlungwitz, pp 192–218

  13. Tewes A, Wappler A, Peschke EM, Garve R, Nover L (1982) Z Pflanzenphysiol 106: 311–324

  14. Tisserat B, Esan E, Murashige T (1979) Hort Rev 1: 1–78

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Correspondence to Juan Segura.

Additional information

Communicated by M. H. Zenk

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Arrillaga, I., Brisa, M.C. & Segura, J. Somatic embryogenesis from hypocotyl callus cultures of Digitalis obscura L.. Plant Cell Reports 6, 223–226 (1987). https://doi.org/10.1007/BF00268485

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  • Agar
  • Liquid Medium
  • Growth Regulator
  • Somatic Embryogenesis
  • Normal Development