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Expression of the L11-L1 operon in mutants of Escherichia coli lacking the ribosomal proteins L1 or L11

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Summary

Using a variety of immunological techniques, the supernatant levels of ribosomal proteins were measured in mutants lacking the ribosomal proteins L1 or L11, and in wild-type strains. There was a 2.5–5-fold elevation of protein L11 level in the supernatant of strains lacking protein L1, compared to wild-type. In contrast, there was no elevation, but rather a diminution, in the corresponding L1 level in strains lacking protein L11, compared to wild-type. These results are consistent with a model for the control of expression of the L11-L1 operon in which protein L1 is an inhibitor of expression of that operon, but protein L11 is not. The supernatant concentrations of other proteins were indistinguishable in all strains.

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Correspondence to Georg Stöffler.

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Communicated by E. Bautz

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Stöffler, G., Hasenbank, R. & Dabbs, E.R. Expression of the L11-L1 operon in mutants of Escherichia coli lacking the ribosomal proteins L1 or L11. Molec. Gen. Genet. 181, 164–168 (1981). https://doi.org/10.1007/BF00268422

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Keywords

  • Escherichia Coli
  • Ribosomal Protein
  • Immunological Technique
  • Supernatant Concentration
  • Supernatant Level