β-Glucosidase was immobilized in calcium alginate gel using genipin as the cross-linking reagent. The activity of this immobilized enzyme with genipin cross-linking was higher than that with other cross-linking reagents and no decrease in activity was observed even after using the gel 12 times.
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Fujikawa S, Nakamura S, Koga K (1987a) Preparation of immobilized enzyme with genipin as a crosslinking reagent. Abstract, Annual meeting of Agricultural Chemical Society of Japan, April 3, 1987, Tokyo, Japan, p 377
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Fujikawa, S., Yokota, T. & Koga, K. Immobilization of β-glucosidase in calcium alginate gel using genipin as a new type of cross-linking reagent of natural origin. Appl Microbiol Biotechnol 28, 440–441 (1988). https://doi.org/10.1007/BF00268210
- Immobilize Enzyme