Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli

Summary

The replication of an F′ plasmid in a dnaC mutant, thermolabile for initiation of chromosomal replication, has been re-examined using a novel DNA-DNA annealing assay. Plasmid replication ceases rapidly at non-permissive conditions, consistent with a direct role for the dnaC product in the replication of F.

This is a preview of subscription content, log in to check access.

References

  1. Bazaral, M., Helinski, D.R.: Replication of a bacterial plasmid and an episome in Escherichia coli. Biochemistry 9, 399–406 (1970)

  2. Bollum, F.J.: In G.L. Cantoni and D.R. Davies (eds.), Procedures in nucleic acid research, pp. 296–300. N.Y.C.: Harper and Row 1966

  3. Bukhari, A.I., Metlay M.: Genetic mapping of prophage Mu. Virology 54, 109–116 (1973)

  4. Carl, P.L.: Escherichia coli mutants with temperature-sensitive synthesis of DNA. Molec. gen. Genet. 109, 107–122 (1970)

  5. Clark, D.J., Maaløe, O.: DNA replication and the division cycle in Escherichia coli. J. molec. Biol. 23, 99–112 (1967)

  6. Clewell, D.B., Helinski, D.R.: Properties of a supercoiled deoxyribonucleic acid-protein complex and strand specificity of the relaxation event. Biochemistry 9, 4428–4440 (1970)

  7. Clowes, R.C.: Molecular structure of bacterial plasmids. Bact. Rev. 36, 361–405 (1972)

  8. Collins, J., Pritchard, R.H.: Relationship between chromosome replication and F′lac episome replication in Escherichia coli. J. molec. Biol. 78, 143–155 (1973)

  9. Cooper, S.: Relation of F′lac replication and chromosome replication. Proc. nat. Acad. Sci. (Wash.) 69, 2706–2710 (1972)

  10. Daniell, E., Abelson, J., Kim, J.S., Davidson, N.: Heteroduplex structures of bacteriophage Mu DNA. Virology 51, 237–239 (1973)

  11. Davis, D.B., Helmstetter, C.E.: Control of F′lac replication in Escherichia coli B/r. J. Bact. 114, 294–299

  12. Denhardt, D.T.: A membrane-filter technique for the detection of complementary DNA. Biochem. biophys. Res. Commun. 23, 641–646 (1966)

  13. Falkow, S., Citarella, R.V.: Molecular homology of F-merogenote DNA. J. molec. Biol. 12, 138–151 (1965)

  14. Frame, R., Bishop, J.O.: The number of sex-factors per chromosome in Escherichia coli. Biochem. J. 121, 93–103 (1971)

  15. Freifelder, D., Folkmanis, A., Kirschner, I.: Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J. Bact. 105, 722–727 (1971)

  16. Goebel, W.: The influence of dnaA and dnaC mutations on the initiation of plasmid DNA replication. Biochem. biophys. Res. Commun. 51, 1000–1007 (1973)

  17. Goebel, W., Schrempf, H.: Replication of plasmid DNA in temperature sensitive DNA replication mutants of Escherichia coli. Biochim. biophys. Acta (Amst.) 262, 32–41 (1972)

  18. Helmstetter, C.E.: Rate of DNA synthesis during the division cycle of Escherichia coli B/r. J. molec. Biol. 24, 417–427 (1967)

  19. Hiraga, S., Saitoh, T.: Initiation of DNA replication in Escherichia coli. II. Effect of rifampicin on the resumption of replication of F episome and chromosome upon the returning of dna mutants from a non-permissive to a permissive temperature. Molec. gen. Genet. 137, 239–248 (1973)

  20. Kline, B.C.: Mechanism and biosynthetic requirements for F plasmid replication in Escherichia coli. Biochemistry 13, 139–146 (1974)

  21. Kuempel, P.: DNA-DNA hybridization assay for replication origin DNA of Escherichia coli. J. Bact. 110, 917–925 (1972)

  22. Martuscelli, J., Taylor, A.L., Cummings, D.J. Chapman, V.V., DeLong, S.S., Canedo, L.: Electron microscopic evidence for linear insertion of bacteriophage Mu-1 in lysogenic bacteria. J. Virol. 8, 551–563 (1971)

  23. Pritchard, R.H., Chandler, M.G., Collins, J.: Independence of F replication and chromosome replication in Escherichia coli. Molec. gen. Genet. 138, 143–155 (1975)

  24. Thompson, R., Broda, P.: DNA polymerase III and the replication of F and ColVBtrp in Escherichia coli K-12. Molec. gen. Genet. 127, 255–258 (1973)

  25. Waggoner, B.T., Gonzalez, N.S., Taylor, A.L.: Isolation of heterogeneous circular DNA from induced lysogens of bacteriophage Mu-1. Proc. nat. Acad. Sci. (Wash.) 71, 1255–1259 (1974)

  26. Wechsler, James A.: Genetic and phenotypic characterization of dnaC mutation. J. Bact. 121, 595–599 (1975)

  27. Zeuthen, J., Pato, M.L.: Replication of the F′lac sex factor in the cell cycle of Escherichia coli. Molec. gen. Genet. 111, 242–255 (1971)

Download references

Author information

Additional information

Communicated by G. O'Donovan

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Van Brunt, J., Waggoner, B.T. & Pato, M.L. Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli . Molec. Gen. Genet. 150, 285–292 (1977). https://doi.org/10.1007/BF00268127

Download citation

Keywords

  • Escherichia Coli
  • Direct Role
  • Plasmid Replication
  • Chromosomal Replication
  • Annealing Assay