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Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli


The replication of an F′ plasmid in a dnaC mutant, thermolabile for initiation of chromosomal replication, has been re-examined using a novel DNA-DNA annealing assay. Plasmid replication ceases rapidly at non-permissive conditions, consistent with a direct role for the dnaC product in the replication of F.

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Communicated by G. O'Donovan

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Van Brunt, J., Waggoner, B.T. & Pato, M.L. Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli . Molec. Gen. Genet. 150, 285–292 (1977). https://doi.org/10.1007/BF00268127

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  • Escherichia Coli
  • Direct Role
  • Plasmid Replication
  • Chromosomal Replication
  • Annealing Assay