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The construction in vitro of transducing derivatives of phage lambda


Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropriate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, supF, hsd, tna and lig genes have been isolated.

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Communicated by B.A. Bridges

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Borck, K., Beggs, J.D., Brammar, W.J. et al. The construction in vitro of transducing derivatives of phage lambda. Molec. Gen. Genet. 146, 199–207 (1976).

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  • Enzyme
  • Restriction Enzyme
  • General Property
  • Phage Lambda
  • Receptor Genome