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Contrôle biochimique de la récombinaison chez Saccharomyces cerevisiae

I. Inhibition par la L-histidine de la récombinaison intragénique mitotique au locus ad3

Biochemical control of recombination in Saccharomyces cerevisiae

I. L-histidine inhibition of intragenic mitotic recombination at the ad 3 locus

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Summary

A yeast strain heteroallelic at the ad 3 locus is used to study mitotic intragenic recombination. L-histidine inhibits the recombination at this locus in strains heteroallelic for all possible combinations between the four alleles studied. No other amino acid has this effect. The kinetic of recombination was studied by addition of L-histidine at different times or by compeating the L-histidine present with D-histidine added at different times. The two techniques gave similar results showing that the recombination takes place between the 8th and 24th hour after plating although it is expressed a few days later.

Taking advantage of the early interval in which the recombination takes place and of the fact than the “petite” mutation is induced by acriflavine only in new formed buds, we developed a technique to study the recombination in liquid medium, thanks to which, we were able to show that L-histidine inhibits the genetic event itself.

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Bibliographie

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Communiqué par E. Magni

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Luzzati, M., Clavilier, L., Pere-Aubert, G. et al. Contrôle biochimique de la récombinaison chez Saccharomyces cerevisiae . Molec. Gen. Genet. 111, 120–137 (1971). https://doi.org/10.1007/BF00267787

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