Molecular and General Genetics MGG

, Volume 109, Issue 4, pp 338–355 | Cite as

Morphogenesis of the tail fiber of bacteriophage T4

II. Isolation and characterization of a component (the genes 35-36-37-38 directing half fiber)
  • Sumi Imada
  • Akira Tsugita


The tail fiber component ofcoli phage T4 was purified and partially characterized. The material was purified approximately 1 200 fold over the original lysate obtained fromE. coli B/1 cells infected with a mutant in gene 34 (am A455). The purified material was ultracentrifugally, electrophoretically and electron microscopically homogeneous. Its chemical composition were also analyzed.

The purified component was characterized to be a half fiber controlled by at least four genes, 35, 36, 37, and 38.


Fiber Component Tail Fiber Original Lysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Brenner, S., Streisinger, G., Hone, R. W., Champe, S. P., Barnet, L., Benzer, S., Rees, M. W.: Structural components of bacteriophage. J. molec. Biol.1, 281–292 (1959).CrossRefGoogle Scholar
  2. Brückner, J.: Estimation of monosacharides by the orcinol-sulphuric acid reaction. Biochem. J.60, 200–206 (1955).CrossRefGoogle Scholar
  3. Davis, B. J.: Disk electrophoresis-II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci.121, Art. 2, 404–427 (1964).CrossRefGoogle Scholar
  4. Edgar, R. S., Denhardt, G. H., Epstein, R. H.: A comparative genetic study of conditional lethal mutations of bacteriophage T4D. Genetics49, 635–648 (1964).PubMedPubMedCentralGoogle Scholar
  5. — Leilausis, I.: Serological studies with mutants of phage T4D defective in genes determining tail fiber structure. Genetics52, 1187–1200 (1965).PubMedPubMedCentralGoogle Scholar
  6. — Wood, W. B.: Morphogenesis of bacteriophage T4 in extructs of mutant-infected cells. Proc. nat. Acad. Sci. (Wash.)55, 498–505 (1966).CrossRefGoogle Scholar
  7. Eiserling, F. A., Epstein, R. H.: Electron microscopic study of the structure of mutants of bacteriophage T4D defective in tail fiber genes. Virology33, 405–412 (1967).CrossRefGoogle Scholar
  8. Epstein, R. H., Bolle, A., Steinberg, C. M., Kellenberger, E., Boy de la Tour, E., Chevalley, R.: Physiological studies of conditional lethal mutants of bacteriophage T4D. Cold Spr. Harb. Symp. quant. Biol.28, 375–394 (1963).CrossRefGoogle Scholar
  9. Fukami, A., Adachi, A.: On a new preparation method of a self perforated micro plastic grid and its apprication I. J. Electronmicroscopy14, 112–118 (1965).Google Scholar
  10. Goodwin, T. W., Morton, R. A.: The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochem. J.40, 628–631 (1946).CrossRefGoogle Scholar
  11. Hirs, C. H. W.: The oxidation of ribonuclease with performic acid. J. biol. Chem.219, 611–621 (1956).PubMedGoogle Scholar
  12. Inouye, M., Tsugita, A.: The amino acid sequence of T4 bacteriophage lysozyme. J. molec. Biol.22, 193–196 (1966).CrossRefGoogle Scholar
  13. Kellenberger, E., Bolle, A., Boy de la Tour, E., Epstein, R. H., Franklin, N. C., Jerne, N. K., Reale-Scafat A., Sehaud, J., Bendet, J. I., Goldstein, D., Lauffer, M. A.: Functions and properties related to the tail fibers of bacteriophage T4. Virology26, 419–440 (1965).CrossRefGoogle Scholar
  14. King, J., Wood, W. B.: Assembly of bacteriophage T4 tail fibers: The sequence of gene product interaction. J. molec. Biol.39, 583–601 (1969).CrossRefGoogle Scholar
  15. Lowry, O. H., Rosenbrough, A. L., Farr, N. L., Rendall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem.193, 265–275 (1951).PubMedGoogle Scholar
  16. Ornstein, L.: Disk electrophoresis—I. Background and theory. Ann. N. Y. Acad. Sci.121, Art. 2, 321–349 (1964).CrossRefGoogle Scholar
  17. Schachmann, H. K.: Techniques for characterization of proteins: Ultracentrifugation, diffusion, and viscometry. Meth. in Enzymol.,4, 32–103 (1959).CrossRefGoogle Scholar
  18. Shinomiya, T., Imada, S., Tsugita, A.: Isolation of the gene 35-product and the genes 36-37-38 product of the fiber component of T4 phage (in preparation).Google Scholar
  19. Simon, L. D., Anderson, T. F.: The infection ofEscherichia coli by T2 and T4 bacteriophages as seen in electron microscope. I. Attachment and penetration. Virology32, 279–297 (1967).CrossRefGoogle Scholar
  20. — II. Structure and function of the baseplate. Virology32, 298–305 (1967).CrossRefGoogle Scholar
  21. Takata, R., Imada, S., Shinomiya, T., Tsugita, A.: Purification of the tail fiber components of bacteriophage T4. 7th of the Japanese Biophysical Congress, Tokyo (1968).Google Scholar
  22. —, Tsugita, A.: Morphogenesis of the tail fiber of bacteriophage T4. I. Isolation and characterization of a component (the gene 34 product). J. molec. Biol.54, 45–57 (1970).CrossRefGoogle Scholar
  23. Terzaghi, E., Tsugita, A.:In vitro assembly of T4 phage. Japan J. Genetics41, 484 (Abstr.) (1966).Google Scholar
  24. Ward, S., Wood, W. B.: Purification of an intermediate in phage T4 tail fiber assembly. Fed. Proc.27, 592 (1968).Google Scholar
  25. Wood, W. B., Henninger, M.: Attachment of tail fibers in bacteriophage T4 assembly: some properties of the reactionin vitro and its genetic control. J. molec. Biol.39, 603–618 (1969).CrossRefGoogle Scholar
  26. Yamakawa, T., Ueta, N.: Gas-liquid chromatography of carbohydrates. Jap. J. exp. Med.34, 37–51 (1964).PubMedGoogle Scholar
  27. Yanagida, M., Ahmad-Zadeh, C.: Determination of gene product positions in bacteriophage T4 by specific antibody association. Submitted to J. molec. Biol. (1970).Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • Sumi Imada
    • 1
  • Akira Tsugita
    • 1
  1. 1.Laboratory of Molecular Genetics, Medical SchoolUniversity of OsakaOsakaJapan

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